SYK 抗体 (pTyr525, pTyr526)
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- 抗原 See all SYK 抗体
- SYK (Spleen tyrosine Kinase (SYK))
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抗原表位
- pTyr525, pTyr526
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This SYK antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- 特异性
- This antibody detects Syk pTyr525/526.
- 纯化方法
- Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
- 免疫原
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Tyr525/526 of human Syk.
- 亚型
- Ig Fraction
- Top Product
- Discover our top product SYK Primary Antibody
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anti-Spleen tyrosine Kinase (SYK) (AA 5-360) antibody
SYK 适用: 人, 小鼠, 大鼠 WB, IHC (p), IP, ICC 宿主: 小鼠 Monoclonal SYK-01 unconjugated
anti-Spleen tyrosine Kinase (SYK) (pTyr323) antibodySYK 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Spleen tyrosine Kinase (SYK) (pTyr525) antibodySYK 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Spleen tyrosine Kinase (SYK) (pTyr348) antibodySYK 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Spleen tyrosine Kinase (SYK) (Internal Region) antibodySYK 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p), IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Spleen tyrosine Kinase (SYK) (AA 217-356) antibodySYK 适用: 人 WB, ELISA, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 2G11E6 unconjugated
anti-Spleen tyrosine Kinase (SYK) (AA 387-417), (C-Term) antibodySYK 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB03097 unconjugated
anti-Spleen tyrosine Kinase (SYK) (pTyr525), (pTyr526) antibodySYK 适用: 人, 小鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Spleen tyrosine Kinase (SYK) (pTyr323) antibodySYK 适用: 小鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Spleen tyrosine Kinase (SYK) (pTyr323) antibodySYK 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.25 mg/mL
- 缓冲液
- PBS with 0.09 % (W/V) Sodium Azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- 抗原
- SYK (Spleen tyrosine Kinase (SYK))
- 别名
- SYK (SYK 产品)
- 别名
- p72-Syk antibody, Sykb antibody, p72syk antibody, spleen associated tyrosine kinase antibody, spleen tyrosine kinase antibody, syk antibody, SYK antibody, Syk antibody
- 背景
- Syk is a positive effector of BCR-stimulated responses. This protein couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C-gamma-dependent pathway, when phosphorylated on Tyr-342 and Tyr-346. Thus the differential phosphorylation of Syk can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ion.Synonyms: Spleen tyrosine kinase, Tyrosine-protein kinase SYK
- 分子量
- 72066 Da
- 基因ID
- 6850, 9606
- UniProt
- P43405
- 途径
- Fc-epsilon Receptor Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Thromboxane A2 Receptor Signaling, BCR Signaling
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