MAPKAP Kinase 5 抗体 (Internal Region)
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- 抗原 See all MAPKAP Kinase 5 (MAPKAPK5) 抗体
- MAPKAP Kinase 5 (MAPKAPK5) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5))
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抗原表位
- AA 192-206, Internal Region
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适用
- 人, 小鼠, 大鼠, Pig, Cow, 犬
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This MAPKAP Kinase 5 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
- 序列
- C-PQVLEAQRRH QKEKS
- 特异性
- Recognizes MK5 / MAPKAPK5 (192-206).
- 纯化方法
- Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
- 免疫原
- Peptide with sequence from the internal region (192-206) of the protein sequence according to NP_003659.2 NP_620777.1.
- Top Product
- Discover our top product MAPKAPK5 Primary Antibody
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anti-Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5) (pThr182) antibody
MAPKAPK5 适用: 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5) (AA 371-471) antibodyMAPKAPK5 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 3D7 unconjugated
anti-Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5) (pThr182) antibodyMAPKAPK5 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5) antibodyMAPKAPK5 适用: 人 WB, ELISA, IHC 宿主: 小鼠 Monoclonal 7H10B4 unconjugated
anti-Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5) (pSer93) antibodyMAPKAPK5 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5) (AA 1-471) antibodyMAPKAPK5 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5) (AA 1-471) antibodyMAPKAPK5 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5) (AA 371-471) antibodyMAPKAPK5 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2D5 unconjugated
anti-Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5) (AA 1-471) antibodyMAPKAPK5 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5) (Internal Region) antibodyVerified MAPKAPK5 适用: 人, 小鼠 WB, ELISA, IHC, IP 宿主: 山羊 Polyclonal unconjugated
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- 应用备注
- Peptide ELISA: 1/64000 (Detection Limit). Western blot: 0.5-1 μg/mL. In transfected HEK293 transiently expressing either Mouse MK5 fused to GFP or the similar Mouse MK2 fused to GFP only the expected band was observed in the MK5 lane after AP32809PU-N labeling. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Immunoprecipitation: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced wiith Mk5/Erk3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5 μg AP32808PU-N or AP32809PU-N. Western blots of the Immunoprecipitation were labelled with Mouse anti-MK5 (SC) or with Rabbit anti-ERK3 serum (CST). An approx 55 kDa of MK5 is only precipitated from lysates of those KO MEFs that have been rescued by the ERK3/MK5 expression constructas described in PMID: 22508986. Immunohistochemistry on Paraffin Sections: 4-8 μg/mL. In paraffin embedded Human Kidney shows nuclear staining in the DCT.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Tris saline, pH ~7.3, 0 Preservatives: 0.02 % Sodium Azide Stabilizers: 0.5 % BSA
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
- 有效期
- 12 months
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- 抗原
- MAPKAP Kinase 5 (MAPKAPK5) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5))
- 别名
- mapkap kinase-5 (MAPKAPK5 产品)
- 别名
- MAPKAPK5 antibody, MK5 antibody, PRAK antibody, MAPKAP-K5 antibody, MK-5 antibody, mitogen-activated protein kinase-activated protein kinase 5 antibody, MAP kinase-activated protein kinase 5 antibody, MAPKAPK5 antibody, mapkapk5 antibody, Mapkapk5 antibody
- 途径
- MAPK Pathway
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