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HGF 抗体 (C-Term)

Name: HGF 抗体 (C-Term)
SKU: ABIN5536237
Availability: OutOfStock

HGF 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal unconjugated

Antibody is used in Western blot to detect HGF in Ramos tissue lysate. (HGF 抗体 (C-Term))

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HGF antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (HGF 抗体 (C-Term))

IHC

Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram). (HGF 抗体 (C-Term))

FACS

3 images

产品编号 ABIN5536237

发货至: 中国

Datasheet as PDF

抗原 See all HGF 抗体

HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))
抗原表位
15

11

7

6

3

3

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1
AA 521-554, C-Term
适用
77

33

32

16

8

4

3

3

2

2

2

2

1

1
人
宿主
66

22

4

1
兔
Compatible Secondaries
克隆类型
72

21
多克隆
标记
48

13

9

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1
This HGF antibody is un-conjugated
应用范围
84

33

26

14

13

13

12

10

8

6

3

3

2

2

2

1

1

1

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

纯化方法

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

免疫原

This HGF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 521-554 amino acids from the C-terminal region of human HGF.

亚型

Ig Fraction

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anti-Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 1-728) antibody
HGF 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated

anti-Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (N-Term) antibody
HGF 适用: 人, 大鼠, 小鼠, 犬, 绵羊, 兔, Cow, 马, 豚鼠, Pig, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 521-554) antibody
HGF 适用: 人 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated

anti-Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 201-300) antibody
HGF 适用: 人, 大鼠, 小鼠, 绵羊 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 482-710) antibody
HGF 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 306-471) antibody
HGF 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) antibody
HGF 适用: 人, 小鼠 WB, IHC (p) 宿主: 小鼠 Monoclonal 7-2 unconjugated

anti-Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) antibody
HGF 适用: 人, 大鼠, 小鼠 IHC 宿主: 兔 Polyclonal unconjugated

anti-Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 216-265) antibody
HGF 适用: 人, 大鼠, 小鼠, 犬, 兔, Cow, 马, 豚鼠, 猴, Hamster WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 521-554) antibody
HGF 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal unconjugated

应用备注

For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50

For IHC-P starting dilution is: 1:50~100

限制

仅限研究用
状态

Liquid

浓度

2 mg/mL

缓冲液

Supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
抗原

HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))

别名

HGF (HGF 产品)

别名

DFNB39 antibody, F-TCF antibody, HGFB antibody, HPTA antibody, SF antibody, HGF antibody, C230052L06Rik antibody, HGF/SF antibody, NK1 antibody, NK2 antibody, SF/HGF antibody, hepatocyte growth factor antibody, HGF antibody, Hgf antibody

背景

Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69- kDa alpha-chain and 34- kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms. Transcript Variant: This variant (1) encodes the longest isoform (1). To date, experimental evidence for cleavage of the proprotein into two mature chains has been shown only for isoform 1.

分子量

83 kDa

基因ID

3082

UniProt

P14210

途径

RTK signaling, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor