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HGF 抗体 (N-Term)

Name: HGF 抗体 (N-Term)
SKU: ABIN2781816
Availability: OutOfStock

HGF 适用: 人, 大鼠, 小鼠, 犬, 绵羊, 兔, Cow, 马, 豚鼠, Pig, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated

WB Suggested Anti-HGF Antibody Titration: 0.

Immunohistochemistry (IHC) image for anti-Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (N-Term) antibody (ABIN2781816)

IHC

Host: Rabbit Target Name: HGF Sample Tissue: Human HepG2 cell Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration: 1 µg/mL Peptide Concentration: 5.

Host: Rabbit Target Name: HGF Sample Type: 721_B Cell lysates Antibody Dilution: 1.

Host: Rabbit Target Name: HGF Sample Tissue: Human HepG2 Antibody Dilution: 1.

Host: Rabbit Target Name: HGF Sample Type: 293T Whole Cell lysates Antibody Dilution: 0.

Host: Rabbit Target Name: HGF Sample Type: 721_B Whole Cell lysates Antibody Dilution: 0.

8 images

(5 references)

产品编号 ABIN2781816

发货至: 中国

Datasheet as PDF

抗原 See all HGF 抗体

HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))
抗原表位
15

11

6

6

3

3

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1
N-Term
适用
76

32

31

15

7

3

2

2

2

2

1

1

1
人, 大鼠, 小鼠, 犬, 绵羊, 兔, Cow, 马, 豚鼠, Pig, 斑马鱼
宿主
65

22

4

1
兔
Compatible Secondaries
克隆类型
71

21
多克隆
标记
47

13

9

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1
This HGF antibody is un-conjugated
应用范围
83

32

26

14

13

13

12

10

8

6

3

3

2

2

2

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC)

序列

VKKEFGHEFD LYENKDYIRN CIIGKGRSYK GTVSITKSGI KCQPWSSMIP

特异性

100 % homologous to all 6 isoforms, 1 (83 kDa), 2 (34 kDa), 3 (83 kDa), 4 (35 kDa), 5 (33 kDa), 6 (24 kDa)

预测反应

Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 80%

产品特性

This is a rabbit polyclonal antibody against HGF. It was validated on Western Blot using a cell lysate as a positive control.

纯化方法

Affinity Purified

免疫原

The immunogen is a synthetic peptide directed towards the N terminal region of human HGF

Top Product

Discover our top product HGF Primary Antibody
anti-Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 1-728) antibody
HGF 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated

anti-Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 521-554) antibody
HGF 适用: 人 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated

anti-Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 201-300) antibody
HGF 适用: 人, 大鼠, 小鼠, 绵羊 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 482-710) antibody
HGF 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 306-471) antibody
HGF 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) antibody
HGF 适用: 人, 小鼠 WB, IHC (p) 宿主: 小鼠 Monoclonal 7-2 unconjugated

anti-Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) antibody
HGF 适用: 人, 大鼠, 小鼠 IHC 宿主: 兔 Polyclonal unconjugated

anti-Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 216-265) antibody
HGF 适用: 人, 大鼠, 小鼠, 犬, 兔, Cow, 马, 豚鼠, 猴, Hamster WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 521-554) antibody
HGF 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal unconjugated

anti-Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 30-285) antibody
HGF 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

应用备注

Optimal working dilutions should be determined experimentally by the investigator.

说明

Antigen size: 728 AA

限制

仅限研究用
状态

Liquid

浓度

Lot specific

缓冲液

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and may contain up to 2 % sucrose.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freeze-thaw cycles.

储存条件

-20 °C

储存方法

For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Nejak-Bowen, Orr, Bowen, Michalopoulos: "Conditional genetic elimination of hepatocyte growth factor in mice compromises liver regeneration after partial hepatectomy." in: PLoS ONE, Vol. 8, Issue 3, pp. e59836, (2013) (PubMed).

Ji, Zhang, You, Li, Liu, Jiang, Liu, Zhang, Wang, Tao, Dou: "The differentiation of MSCs into functional hepatocyte-like cells in a liver biomatrix scaffold and their transplantation into liver-fibrotic mice." in: Biomaterials, Vol. 33, Issue 35, pp. 8995-9008, (2012) (PubMed).

Zampell, Avraham, Yoder, Fort, Yan, Weitman, Mehrara: "Lymphatic function is regulated by a coordinated expression of lymphangiogenic and anti-lymphangiogenic cytokines." in: American journal of physiology. Cell physiology, Vol. 302, Issue 2, pp. C392-404, (2012) (PubMed).

Sen, Peng, Saigal, Williams, Johnson: "Distinct interactions between c-Src and c-Met in mediating resistance to c-Src inhibition in head and neck cancer." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 17, Issue 3, pp. 514-24, (2011) (PubMed).

Williams, Mehta, Peyton, Zeiger, Van Vliet, Griffith: "Autocrine-controlled formation and function of tissue-like aggregates by primary hepatocytes in micropatterned hydrogel arrays." in: Tissue engineering. Part A, Vol. 17, Issue 7-8, pp. 1055-68, (2011) (PubMed).
抗原

HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))

别名

HGF (HGF 产品)

别名

DFNB39 antibody, F-TCF antibody, HGFB antibody, HPTA antibody, SF antibody, HGF antibody, C230052L06Rik antibody, HGF/SF antibody, NK1 antibody, NK2 antibody, SF/HGF antibody, hepatocyte growth factor antibody, HGF antibody, Hgf antibody

背景

Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69- kDa alpha-chain and 34- kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity.Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69- kDa alpha-chain and 34- kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms.
Alias Symbols: F-TCF, HGFB, HPTA, SF, DFNB39
Protein Interaction Partner: ADAMTSL4, MEOX2, MET, F11, ST14, HGFAC, LCN2, KLKB1, VTN, PLAU, CLEC3B, SDC1, SDC2, HPN, HGF, FN1, NRP1,
Protein Size: 728

分子量

83 kDa

基因ID

3082

NCBI登录号

NM_001010932, NP_001010932

UniProt

P14210

途径

RTK signaling, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor