Western Blotting (WB), ELISA, Immunofluorescence (IF)
原理
Rabbit polyclonal antibody raised against synthetic phosphopeptide of AURKB.
特异性
Reactivity occurs against human Aurora Kinase B pT232 protein and This antibody is specific to the phosphorylated form of the protein. Reactivity with non-phosphorylated human Aurora Kinase B is minimal by ELISA. No reaction is expected against Aurora Kinase A.
交叉反应
Chimpanzee, Cow, 犬, 人, 小鼠, Pig, 大鼠
产品特性
Antibody Reactive Against Synthetic Peptide.
免疫原
Synthetic phosphopeptide corresponding to residues surrounding T232 of human AURKB.
ELISA (1:10000-1:50000) Western Blot (1:250-1:2000) The optimal working dilution should be determined by the end user.
限制
仅限研究用
状态
Liquid
缓冲液
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01 % sodium azide)
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C,-20 °C
储存方法
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Yasui, Urano, Kawajiri, Nagata, Tatsuka, Saya, Furukawa, Takahashi, Izawa, Inagaki: "Autophosphorylation of a newly identified site of Aurora-B is indispensable for cytokinesis." in: The Journal of biological chemistry, Vol. 279, Issue 13, pp. 12997-3003, (2004) (PubMed).
Kimura, Uchida, Takano, Kitagawa, Okano: "Cell cycle-dependent regulation of the human aurora B promoter." in: Biochemical and biophysical research communications, Vol. 316, Issue 3, pp. 930-6, (2004) (PubMed).
Gruneberg, Neef, Honda, Nigg, Barr: "Relocation of Aurora B from centromeres to the central spindle at the metaphase to anaphase transition requires MKlp2." in: The Journal of cell biology, Vol. 166, Issue 2, pp. 167-72, (2004) (PubMed).