p53 抗体 (AA 293-322)
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北京 101111
Quick Overview for p53 抗体 (AA 293-322) (ABIN3029384)
抗原
See all p53 (TP53) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 293-322
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原理
- p53 Antibody / TP53
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预测反应
- Primate
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纯化方法
- Antigen affinity purified
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免疫原
- A portion of amino acids 293-322 from the human protein was used as the immunogen for this TP53 Antibody for Immunofluorescence / Tumor Protein p53 IF Antibody.
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亚型
- Ig Fraction
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anti-Tumor Protein P53 (TP53) antibody
KO Validated TP53 适用: 人 WB, IF, IP, IHC (p), ICC, ChIP 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (N-Term) antibodyTP53 适用: 人 WB, IF, IP, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (AA 301-393) antibodyTP53 适用: 人 WB, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) antibodyTP53 适用: 人, 大鼠, 小鼠, 猴 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (AA 293-322) antibodyTP53 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB7777 unconjugated
anti-Tumor Protein P53 (TP53) (AA 94-201) antibodyTP53 适用: 人 WB, ELISA, IF, IP, IHC (p) 宿主: 小鼠 Monoclonal 2C3 unconjugated
anti-Tumor Protein P53 (TP53) (AA 94-201) antibodyTP53 适用: 人 WB, ELISA, IHC (p), PLA, RNAi 宿主: 小鼠 Monoclonal 2C11 unconjugated
anti-Tumor Protein P53 (TP53) (AA 251-310) antibodyTP53 适用: 人 WB, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (AA 1-393) antibodyTP53 适用: 人 WB, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (pSer46) antibodyTP53 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 36 unconjugated
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应用备注
- Titration of the TP53 Antibody for Immunofluorescence / Tumor Protein p53 IF Antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- In 1X PBS, pH 7.4, with 0.09 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze-thaw cycles
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储存条件
- -20 °C
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储存方法
- Aliquot the TP53 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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- p53 (TP53) (Tumor Protein P53 (TP53))
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别名
- p53
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背景
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Tumor protein p53 (TP53) is a sequence-specific transcription factor that functions as a central tumor suppressor regulating DNA damage responses, cell cycle arrest, apoptosis, and genomic stability. A TP53 Antibody for Immunofluorescence enables direct visualization of p53 localization within cells using fluorescence microscopy, allowing researchers to examine nuclear accumulation, intracellular distribution, and dynamic changes in p53 expression at the single-cell level.
TP53 antibody, also referred to as Tumor protein p53 antibody or Cellular tumor antigen p53 antibody in the literature, targets one of the most extensively studied regulatory proteins in cancer biology. The TP53 gene is located on chromosome 17p13.1 and encodes a transcription factor belonging to the p53 family of DNA-binding proteins. The p53 protein contains several functional domains including an N-terminal transcriptional activation domain, a central DNA-binding region responsible for target gene recognition, a tetramerization domain that forms active p53 complexes, and a C-terminal regulatory region that modulates protein stability and DNA interaction.
In unstressed cells, p53 protein levels remain extremely low due to continuous ubiquitination and proteasomal degradation mediated by the E3 ubiquitin ligase MDM2. When cells encounter stress signals such as DNA damage, oncogene activation, oxidative stress, or hypoxia, this degradation pathway is inhibited and p53 becomes stabilized. The stabilized protein rapidly accumulates in the nucleus where it activates transcription of genes including CDKN1A (p21), BAX, and PUMA. A TP53 Antibody for Immunofluorescence allows these stress-induced changes to be visualized through fluorescent nuclear staining that reflects activation of the p53 signaling pathway.
Because many TP53 mutations produce stabilized proteins that accumulate in tumor cell nuclei, cancer cells frequently display strong nuclear fluorescence when stained using a TP53 Antibody for Immunofluorescence. Immunofluorescence imaging therefore provides a powerful method for detecting abnormal p53 accumulation and examining tumor-associated alterations in p53 regulation. In cancer cell lines, p53 staining commonly appears as bright nuclear fluorescence that contrasts with weak or absent signal in cells lacking stabilized protein.
Immunofluorescence microscopy using a TP53 Antibody for Immunofluorescence is particularly valuable for studying the spatial organization of p53 within individual cells. Fluorescent imaging can reveal nuclear enrichment, redistribution following DNA damage, and cell-to-cell variability in p53 expression within heterogeneous populations. These imaging-based approaches allow researchers to monitor p53 pathway activation after chemotherapeutic treatment, radiation exposure, or experimental induction of cellular stress.
Beyond its classical tumor suppressor function, p53 regulates numerous biological processes including metabolism, autophagy, immune signaling, and stem cell homeostasis. The protein interacts with multiple regulatory partners such as MDM2, ATM, ATR, and p300/CBP that influence transcriptional activity and intracellular localization. Because these regulatory interactions often alter nuclear abundance and spatial distribution of p53, fluorescence imaging using a TP53 Antibody for Immunofluorescence provides a valuable tool for studying p53 signaling dynamics and tumor suppressor activity in normal and transformed cells. -
UniProt
- P04637
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途径
- p53 Pathway, MAPK Pathway, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect
抗原
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