DNMT3L 抗体 (N-Term)
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- 抗原 See all DNMT3L (TRDMT1) 抗体
- DNMT3L (TRDMT1) (DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1))
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抗原表位
- AA 1-387, N-Term
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This DNMT3L antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- Detects ~45 kDa protein. No cross-reactivity against DNMT3a or DNMT3B.
- 产品特性
- Synonyms: DNA (cytosine-5)-methyltransferase 3-like
- 纯化方法
- Protein G Chromatography.
- 免疫原
- Fusion protein amino acids 1-387 of Human DNMT3L (accession number BAA95556).
- 克隆位点
- S117-9
- 亚型
- IgG1
- Top Product
- Discover our top product TRDMT1 Primary Antibody
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anti-DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1) (AA 1-386) antibody
TRDMT1 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1) (AA 147-386) antibodyTRDMT1 适用: 人 WB, ELISA, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 2D10H11 unconjugated
anti-DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1) antibodyTRDMT1 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1) (Internal Region) antibodyTRDMT1 适用: 人, 小鼠 WB, ELISA, IHC, IF, IP, ChIP, ICC 宿主: 兔 Polyclonal unconjugated
anti-DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1) (AA 1-386) antibodyTRDMT1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1) (AA 152-164) antibodyTRDMT1 适用: 人 ELISA 宿主: 兔 Polyclonal unconjugated
anti-DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1) (AA 1-234) antibodyTRDMT1 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1) (AA 1-386) antibodyTRDMT1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1) (AA 357-388), (C-Term) antibodyTRDMT1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB02219 unconjugated
anti-DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1) (AA 383-417), (C-Term) antibodyTRDMT1 适用: 小鼠 WB 宿主: 兔 Polyclonal RB50995 unconjugated
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- 应用备注
- Western blot: 1 μg/mL1 μg/mL was sufficient for detection of DNMT3L in 10 μg of COS cell transiently transfectedwith Flag-tagged DNMT3L lysate by colorimetric immunoblot analysis using Goatanti-Mouse IgG: HRP as the secondary antibody. Immunoprecipitation: 1.0-10 μg/mLImmunofluorescence: 1.0-10 μg/mLImmunocytochemistry: 0.1-1.0 μg/mL
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS, pH 7.4 containing 50 % Glycerol as stabilizer and 0.09 % Sodium Azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. - 有效期
- 12 months
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- 抗原
- DNMT3L (TRDMT1) (DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1))
- 别名
- DNMT3L (TRDMT1 产品)
- 别名
- DNMT3L antibody, D6Ertd14e antibody, ecat7 antibody, DNA methyltransferase 3 like antibody, DNA (cytosine-5-)-methyltransferase 3-like antibody, DNMT3L antibody, Dnmt3l antibody
- 背景
- Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue (1-6). Studies show that DNMT3L regulates the activity of DNMT3A and DNMT3B and stimulates their catalytic activities (7). DNMT3L has specifically been linked to the process of carcinogenesis, thru its role in nuclear programming (8).Synonyms: DNA (cytosine-5)-methyltransferase 3-like
- 基因ID
- 29947
- UniProt
- Q9UJW3
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