AKT1 抗体 (pSer473)
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- 抗原 See all AKT1 抗体
- AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))
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抗原表位
- AA 410-490, pSer473
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This AKT1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 原理
- Akt is a-protein kinase consisting of three proteins (Akt1, Akt2 and Akt3) encoded by the AKT gene which is approximately 55 kDa. Akt is localised to the cytoplasm, nucleus and cell membrane and is involved pathways such as RET signalling, regulation of lipid metabolism, insulin signalling-generic cascades, common cytokine receptor gamma-chain family signalling pathways and apoptotic pathways. Akt regulates proliferation, cell survival, growth, angiogenesis and tumor formation. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Akt1 and Akt2 are expressed in all human cell types and Akt 3 is highly expressed in brain, lung and kidney. Mutations in the AKT1 gene can result in breast cancer and colorectal cancer. STJ97726 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous Akt protein which only binds about S473 when S473 is phosphorylated.
- 特异性
- Phospho-Akt (S473) Mouse Monoclonal Antibody (6F8) detects endogenous levels of AKT1
- 产品特性
- Mouse Monoclonal to Phospho-Akt (S473).
- 纯化方法
- The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
- 免疫原
- synthetic peptide derived from Phospho-Akt (S473)
- 克隆位点
- 6F8
- 亚型
- IgG1
- Top Product
- Discover our top product AKT1 Primary Antibody
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anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (C-Term) antibody
AKT1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 122-443) antibodyAKT1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 1-480) antibodyAKT1 适用: 人 WB, ELISA, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 1-480) antibodyAKT1 适用: 人 WB, ELISA, IF, IHC (p), IP 宿主: 小鼠 Monoclonal 2E11 unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pThr450) antibodyAKT1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer124) antibodyAKT1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) antibodyAKT1 适用: 人, 小鼠, 大鼠, 犬, 猴 WB, IHC, IF, IHC (p), FACS 宿主: 小鼠 Monoclonal 3E3 unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) antibodyAKT1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibodyAKT1 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (C-Term) antibodyAKT1 适用: 人, 小鼠, 大鼠, 小鸡 WB, ELISA, IP, FM, IHC (fp) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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WB 1:1000-2000
IHC 1:100-200 - 说明
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Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- Store at -20°C, and avoid repeat freeze-thaw cycles.
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- 抗原
- AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))
- 别名
- Akt (AKT1 产品)
- 别名
- AKT antibody, CWS6 antibody, PKB antibody, PKB-ALPHA antibody, PRKBA antibody, RAC antibody, RAC-ALPHA antibody, Akt antibody, Rac antibody, PKB/Akt antibody, PKBalpha antibody, AKT/PKB antibody, AKT1 antibody, Akt/PKB antibody, Akt1 antibody, CG4006 antibody, D-Akt antibody, DAKT1 antibody, DAKT1/PKB antibody, DAkt antibody, DAkt1 antibody, DPKB antibody, DRAC-PK antibody, DRAC-PK66 antibody, DRAC-PK85 antibody, Dakt antibody, Dakt1 antibody, Dmel\\CG4006 antibody, Dpkb antibody, PKB/AKT antibody, PKB/dAKT antibody, RacPK antibody, akt antibody, akt1 antibody, dAKT antibody, dAKT/dPKB antibody, dAKT1 antibody, dAkt antibody, dAkt/PKB antibody, dAkt1 antibody, dPKB antibody, dakt antibody, dakt1 antibody, l(3)04226 antibody, l(3)89Bq antibody, p-Akt antibody, pAkt antibody, ACT-5 antibody, akt-1 antibody, pkb antibody, v-akt antibody, v-akt1 antibody, xAct antibody, ATAKT1 antibody, F18A8.2 antibody, F18A8_2 antibody, K+ transporter 1 antibody, POTASSIUM TRANSPORTER antibody, AKT serine/threonine kinase 1 antibody, thymoma viral proto-oncogene 1 antibody, CG4006 gene product from transcript CG4006-RE antibody, actin beta antibody, v-akt murine thymoma viral oncogene homolog 1 S homeolog antibody, v-akt murine thymoma viral oncogene homolog 1 antibody, K+ transporter 1 antibody, Serine/threonine-protein kinase akt-1 antibody, AKT1 antibody, Akt1 antibody, ACTB antibody, akt1.S antibody, akt1 antibody, KT1 antibody, akt-1 antibody
- 基因ID
- 207
- UniProt
- P31749
- 途径
- PI3K-Akt Signaling, RTK signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, Warburg Effect
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