ERK2 抗体
Quick Overview for ERK2 抗体 (ABIN470592)
抗原
See all ERK2 (MAPK1) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Detects endogenous levels of total p42 MAP kinase (Erk2) protein. Recognizes p44 MAP kinase (Erk1) in some cell types, although with lower affinity. It does not cross-react with either JNK/ SAPK or p38 MAP kinase. Species cross-reactivity: Human and mouse.
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纯化方法
- Purified
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免疫原
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Synthetic peptide (KLH coupled) derived from the sequence of rat p42 MAP kinase.
Type of Immunogen: Synthetic peptide -
亚型
- IgG1
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应用备注
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Approved: ELISA, IHC, IHC-P, WB (1:2000)
Usage: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Western Blot: 1:2000. Incubate membrane with diluted antibody in 5 % nonfat dry milk, 1X TBS, 0.1 % Tween-20 at 4?C with gentle shaking, overnight. Immunohistochemistry (Paraffin): 1:50. -
说明
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Target Species of Antibody: Rat
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol, 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))
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别名
- MAPK1 / ERK2 / p42
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背景
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Name/Gene ID: MAPK1
Subfamily: MAPK
Family: Protein Kinase
Synonyms: MAPK1, ERK, ERK-2, ERK2, MAP kinase 1, MAPK 2, MAPK2, MAP kinase isoform p42, MAPK 1, p41mapk, PRKM2, MAP kinase 2, p41, p42-MAPK, PRKM1, ERT1, p38, p40, p42MAPK, Protein tyrosine kinase ERK2 -
基因ID
- 5594
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UniProt
- P28482
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途径
- MAPK Pathway, RTK signaling, Apoptosis, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagy, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, BCR Signaling, S100 Proteins
抗原
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