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BDNF 抗体

BDNF 适用: 人, 大鼠, 小鼠 WB, ELISA, Neut 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN465694
发货至: 中国
  • 抗原 See all BDNF 抗体
    BDNF (Brain-Derived Neurotrophic Factor (BDNF))
    适用
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    人, 大鼠, 小鼠
    宿主
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    克隆类型
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    多克隆
    标记
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    This BDNF antibody is un-conjugated
    应用范围
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    Western Blotting (WB), ELISA, Neutralization (Neut)
    特异性
    Human BDNF
    纯化方法
    Immunoaffinity purified
    免疫原
    E.coli derived recombinant Human BDNF.

    Type of Immunogen: Cell extract
    Top Product
    Discover our top product BDNF Primary Antibody
  • 应用备注
    Approved: ELISA (0.5 - 2 μg/mL), Neut (0.54 - 0.81 μg/mL), WB

    Usage: Immunohistochemistry: This antibody stained U-2 OS and U-251 MG cells. The primary antibody was incubated at 2 μg/mL overnight at 4 °C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Cell Profiling group, SciLifeLab Stockholm. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hBDNF (2 μg/mL), a concentration of 0.54-0.81 μg/mL of this antibody is required. ELISA: To detect hBDNF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human BDNF (LS-C104729) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hBDNF. Western Blot: To detect hBDNF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBDNF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
    说明

    Target Species of Antibody: Human

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Reconstitute with sterile water.
    浓度
    Lot specific
    缓冲液
    Lyophilized, PBS,  pH 7.2. Reconstitute with sterile water.
    注意事项
    avoid freeze thaw cycles.
    储存条件
    4 °C,-20 °C
    储存方法
    Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
  • 抗原
    BDNF (Brain-Derived Neurotrophic Factor (BDNF))
    别名
    BDNF (BDNF 产品)
    别名
    ANON2 antibody, BULN2 antibody, bdnf-A antibody, BDNF antibody, bdnf antibody, brain derived neurotrophic factor antibody, brain-derived neurotrophic factor antibody, brain-derived neurotrophic factor L homeolog antibody, brain-derived neurotrophic factor S homeolog antibody, BDNF antibody, Bdnf antibody, bdnf antibody, bdnf.L antibody, bdnf.S antibody
    背景
    Name/Gene ID: BDNF
    Family: NGF Beta

    Synonyms: BDNF, ANON2, Abrineurin, BULN2, Neurotrophin
    基因ID
    627
    UniProt
    P23560
    途径
    RTK signaling, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity
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