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IL12 抗体

IL12 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal unconjugated
产品编号 ABIN465143
发货至: 中国
  • 抗原 See all IL12 抗体
    IL12 (Interleukin 12 (IL12))
    适用
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    小鼠
    克隆类型
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    单克隆
    标记
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    This IL12 antibody is un-conjugated
    应用范围
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    Western Blotting (WB), ELISA
    特异性
    Human IL-12
    纯化方法
    Protein A purified
    免疫原
    CHO derived recombinant Human IL-12.

    Type of Immunogen: Cell extract
    亚型
    IgG1
    Top Product
    Discover our top product IL12 Primary Antibody
  • 应用备注
    Approved: ELISA (2 - 4 μg/mL), WB (0.5 - 1 μg/mL)

    Usage: Immunohistochemistry: This antibody stained PBMC. The primary antibody was incubated at 10 μg/mL for one hour at room temperature followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Protocol and staining provided by Dr. Lauren Binge, Laboratory of Prof. Charles Mackay, Monash Univeristy, Australia. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2-4 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant human IL-12 when used with biotinylated antigen affinity purified anti-human IL-12 (LS-C104425) as the detection antibody at a concentration of approximately 4-8 μg/mL. Western Blot: To detect hIL-12 by Western Blot analysis this antibody can be used at a concentration of 0.50-1 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-12 is 0.5-1 ng/lane, under non-reducing conditions.
    说明

    Target Species of Antibody: Human

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Sterile water
    浓度
    Lot specific
    缓冲液
    Lyophilized from PBS
    注意事项
    Avoid freeze/thaw cycles.
    储存条件
    4 °C,-20 °C
    储存方法
    Store Lyophilized at room temperature up to 1 month
    Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
  • 抗原
    IL12 (Interleukin 12 (IL12))
    别名
    IL12 / IL-12 (IL12 产品)
    别名
    CLMF antibody, CLMF2 antibody, IL-12B antibody, NKSF antibody, NKSF2 antibody, p40 antibody, Il-12b antibody, Il12p40 antibody, Il-12p40 antibody, Il12 antibody, IL-12p40 antibody, IL-12 antibody, LOC100217488 antibody, IL-12p35 antibody, Il-12a antibody, Ll12a antibody, p35 antibody, IL12p40 antibody, IL12B antibody, IL-12 p35 antibody, interleukin 12B antibody, interleukin 12A antibody, interleukin 12b antibody, interleukin 12a antibody, Interleukin 12 level antibody, IL12B antibody, IL12A antibody, Il12b antibody, Il12a antibody, IL12 antibody, il12a antibody
    背景
    Name/Gene ID: IL-12

    Synonyms: IL-12
    途径
    JAK/STAT Signaling, TLR signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
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