Insulin 抗体
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北京 101111
Quick Overview for Insulin 抗体 (ABIN458570)
抗原
See all Insulin (INS) 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Guinea Pig anti Swine insulin
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特异性
- Purified IgG fraction of polyclonal Guinea Pig antiSerum to Swine insulin
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交叉反应 （详细）
- Inter-species cross-reactivity is a normal feature of antibodies to mammalian proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentRations of the reactants and the sensitivity of the assay arrangement. This antiSerum fraction has not been tested for cross-reactivity.
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产品特性
- The reactivity of the antiserum is directed to insulin as tested by ELISA and immunoblotting. No reaction was obtained with other swine serum proteins. The cytochemical grade allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates, in radioimmunoassay, for the production of immunoconjugates with a selected marker, to prepare immunoaffinity adsorbents by coupling to an artificial carrier, in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent, in Western blotting. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used.
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纯化方法
- Purified IgG fraction from antiserum
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免疫原
- Insulin is a hormone produced by the beta cells of the islets of Langerhans in the pancreas. It plays an important role in the metabolism of carbohydrate by its effects on the storage of glycogen in the liver, the conversion of carbohydrate to fat, and the oxidation of glucose by the peripheral tissues. Insulin has a molecular weight of 6,000 and contains two peptide chains linked by two disulphide bonds. The hormone is isolated from porcine pancreas. Freund's complete adjuvant is used in the first step of the immunization procedure.
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亚型
- IgG
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anti-Insulin (INS) (AA 1-110) antibody
INS 适用: 人 IHC, WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Insulin (INS) (AA 46-59) antibodyINS 适用: 人 WB, IHC (p), FACS, IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Insulin (INS) antibodyINS 适用: 人 IHC, WB, IF 宿主: 兔 Monoclonal unconjugated
anti-Insulin (INS) (AA 90-110) antibodyINS 适用: 小鼠 IHC, WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Insulin (INS) (AA 25-110) antibodyINS 适用: 大鼠 IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Insulin (INS) antibodyINS 适用: 人, 小鼠, Pig, Cow, 大鼠, 兔, 豚鼠, 犬, 猴, 小鸡, 驴, 山羊, Hamster, 马, 绵羊, Cat, Avian WB, IHC (p), IHC (fro) 宿主: 山羊 Polyclonal unconjugated
anti-Insulin (INS) antibodyINS 适用: 人 IHC 宿主: 兔 Monoclonal HMV308 unconjugated Recombinant Antibody
anti-Insulin (INS) (AA 25-110) antibodyINS 适用: 人 IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Insulin (INS) antibodyINS 适用: 人, 小鼠, Pig, Cow, 兔 IHC 宿主: 小鼠 Monoclonal rIRDN-805 unconjugated Recombinant Antibody
anti-Insulin (INS) antibodyINS 适用: 人, 小鼠, Pig, Cow, 兔 IHC 宿主: 兔 Monoclonal IRDN-1980R unconjugated Recombinant Antibody
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应用备注
- Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.
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缓冲液
- Purified hyperimmune guinea pig IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- The lyophilized product is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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- Insulin (INS)
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别名
- Insulin
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途径
- NF-kappaB Signaling, RTK signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation
抗原
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