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CHEK1 抗体 (pSer280)

This 兔多克隆 antibody specifically detects CHEK1 in WB. It exhibits reactivity toward 人.

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产品编号 ABIN401536

发货至: 中国

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Quick Overview for CHEK1 抗体 (pSer280) (ABIN401536)

抗原

See all CHEK1 抗体

CHEK1 (Checkpoint Kinase 1 (CHEK1))

适用

人

宿主

兔

克隆类型

多克隆

标记

This CHEK1 antibody is un-conjugated

应用范围

Western Blotting (WB)

Discover our top product CHEK1 Primary Antibody

抗原表位
38

30

20

19

18

7

7

6

5

4

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pSer280

特异性

This antibody detects endogenous levels of Chk1 only when phosphorylated at Serine 280.

纯化方法

Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

免疫原

The antiserum was produced against synthesized phosphopeptide derived from human Chk1 around the phosphorylation site of Serine 280 (V-T-Sp-G-G).
anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibody
CHEK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Checkpoint Kinase 1 (CHEK1) (pSer296) antibody
CHEK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Checkpoint Kinase 1 (CHEK1) (pSer286) antibody
CHEK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Checkpoint Kinase 1 (CHEK1) (pSer301) antibody
CHEK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Checkpoint Kinase 1 (CHEK1) (pSer345) antibody
CHEK1 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Checkpoint Kinase 1 (CHEK1) (Middle Region) antibody
CHEK1 适用: 人, 小鼠, 大鼠, 犬, Cow, 兔, 马, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibody
CHEK1 适用: 小鼠, 小鸡 ELISA, ICC, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Checkpoint Kinase 1 (CHEK1) antibody
CHEK1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Monoclonal 2F2 unconjugated Recombinant Antibody

anti-Checkpoint Kinase 1 (CHEK1) antibody
KD Validated CHEK1 适用: 人 WB, FACS 宿主: 兔 Monoclonal 25GB2890 unconjugated Recombinant Antibody

anti-Checkpoint Kinase 1 (CHEK1) (pSer280) antibody
KD Validated CHEK1 适用: 人 WB, FACS 宿主: 兔 Monoclonal 25GB3390 unconjugated Recombinant Antibody

应用备注

Western Blot: 1/500approx. 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

限制

仅限研究用
浓度

1.0 mg/mL

缓冲液

PBS (without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

-20 °C

储存方法

Store the antibody (in aliquots) at-20 °C.
抗原

CHEK1 (Checkpoint Kinase 1 (CHEK1))

别名

CHK1

背景

One mechanism by which checkpoints maintain the fidelity of cell cycle events is through blocking mitosis in response to unreplicated or damaged DNA. In most species this occurs through inhibiting activation of the cyclic dependent kinase Cdc2, which regulates entry into mitosis. Chk1, a kinase involved in the DNA damage checkpoint response can phosphorylate Cdc25C an activator of Cdc2. It is hypothesized that Chk1 induces serine 216 phosphorylation of Cdc25C and subsequent 14-3-3 binding negatively regulated Cdc25C, thus preventing it from activating Cdc2.Synonyms: CHEK-1, CHEK1, CHK1 checkpoint homolog, Serine/threonine-protein kinase Chk1

基因ID

1111

NCBI登录号

NP_001107593

UniProt

O14757

途径

p53 Pathway, Apoptosis, Cell Division Cycle, DNA Damage Repair