HMGA1 抗体 (Internal Region)
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- 抗原 See all HMGA1 抗体
- HMGA1 (High Mobility Group AT-Hook 1 (HMGA1))
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抗原表位
- Internal Region
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适用
- 人, 小鼠, 大鼠, 犬, Pig, Cow
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This HMGA1 antibody is un-conjugated
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应用范围
- Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- 序列
- c-LASKQEKDGT EK
- 特异性
- This antibody detects HMGA1 (Internal).
- 交叉反应 （详细）
- Species reactivity (expected):Mouse, Rat, Dog, Pig, CowSpecies reactivity (tested):Human
- 纯化方法
- Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
- 免疫原
- Peptide from the internal region of the protein sequence according to NP_665906.1, NP_665908.1, NP_002122.1, NP_665909.1, NP_665912.1, NP_665910.1. Genename: HMGA1
- Top Product
- Discover our top product HMGA1 Primary Antibody
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anti-High Mobility Group AT-Hook 1 (HMGA1) antibody
HMGA1 适用: 人 WB, IHC, IP, IF, ChIP 宿主: 兔 Monoclonal unconjugated
anti-High Mobility Group AT-Hook 1 (HMGA1) (AA 51-107) antibodyHMGA1 适用: 人, 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group AT-Hook 1 (HMGA1) (AA 64-93), (C-Term) antibodyHMGA1 适用: 人 WB, IF, FACS 宿主: 兔 Polyclonal RB17645 unconjugated
anti-High Mobility Group AT-Hook 1 (HMGA1) (AA 64-93) antibodyHMGA1 适用: 人 WB, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group AT-Hook 1 (HMGA1) antibodyHMGA1 适用: 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group AT-Hook 1 (HMGA1) (AA 1-96) antibodyHMGA1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-High Mobility Group AT-Hook 1 (HMGA1) (AA 1-107) antibodyHMGA1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-High Mobility Group AT-Hook 1 (HMGA1) (Internal Region) antibodyVerified HMGA1 适用: 人 IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-High Mobility Group AT-Hook 1 (HMGA1) (AA 9-21) antibodyVerified HMGA1 适用: 人 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-High Mobility Group AT-Hook 1 (HMGA1) (AA 71-101), (C-Term) antibodyHMGA1 适用: 人, 小鼠 WB, FACS, EIA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Peptide ELISA: antibody detection limit dilution 1: 32000. Western blot: Preliminary experiments gave an approx 50 kDa band in Human Testis andOvary lysates after 0.3 μg/mL antibody staining. Please note that currently we cannot findan explanation in the literature for the band we observe given the calculated size of11.0 kDa according to isoform a (also called HMG-I, NP_665906.1, NP_665908.1,NP_665911.1) and 10.0 kDa according to isoform b( (also called HMG-Y, NP_002122.1,NP_665909.1, NP_665910.1, NP_665912.1). The 50 kDa band was successfully blocked byincubation with the immunizing peptide. We would appreciate any feedback from peoplein the field - have any results been reported with other antibodies/lysates? Have anyfurther splice variants/modified forms been reported?Immunohistochemistry: In paraffin embedded Human Placenta shows strong nuclearstaining. Recommended concentration, 4-6 μg/mL.
- 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
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- 抗原
- HMGA1 (High Mobility Group AT-Hook 1 (HMGA1))
- 别名
- HMGA1 / HMGIY (HMGA1 产品)
- 别名
- HMG-R antibody, HMGA1A antibody, HMGIY antibody, Hmgi antibody, Hmgiy antibody, hmga1 antibody, hmgiy antibody, wu:fb12b06 antibody, wu:fb18f11 antibody, wu:fc19f01 antibody, wu:fi38b05 antibody, zgc:85677 antibody, AL023995 antibody, Hmga1a antibody, Hmga1b antibody, Hmgy antibody, HMG-I(Y) antibody, HMG-Y antibody, HMGA1 antibody, zgc:153948 antibody, high mobility group AT-hook 1 antibody, high mobility group protein HMG-I/HMG-Y antibody, high mobility group AT-hook 1a antibody, high mobility group AT-hook 1b antibody, HMGA1 antibody, Hmga1 antibody, TSC_c24220 antibody, hmga1a antibody, LOC705105 antibody, hmga1b antibody
- 背景
- HMGA1 (HMGI/Y) is a non-histone protein involved in many cellular processes, including regulation of inducible gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. There are two splice isoforms of HMGA1 - the full length HMGA1a/HMGI and the shortened form HMGA1b/HMGY. The encoded protein preferentially binds to the minor groove of A+T-rich regions in double-stranded DNA. It has little secondary structure in solution but assumes distinct conformations when bound to substrates such as DNA or other proteins. HMGA1 is frequently acetylated and is found in the nucleus. Interacts with HIV-1 pre-integration complex.Synonyms: High mobility group AT-hook protein 1, High mobility group protein A1, High mobility group protein HMG-I/HMG-Y, High mobility group protein R
- 基因ID
- 3159
- NCBI登录号
- NP_002122
- UniProt
- P17096
- 途径
- Nuclear Hormone Receptor Binding, Autophagy
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