RFP 抗体 (Biotin)
-
- 抗原 See all RFP 抗体
- RFP (Red Fluorescent Protein (RFP))
- 适用
- Discosoma
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This RFP antibody is conjugated to Biotin
-
应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro))
- 交叉反应 （详细）
- Ads. to Hu, Ms, Rt Serum Proteins
- 纯化方法
- Immunoaffinity Chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
- 免疫原
- Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma
- 亚型
- IgG
- Featured
- Discover our best selling RFP Primary Antibody
- Top Product
- Discover our top product RFP Primary Antibody
-
anti-Red Fluorescent Protein (RFP) antibody
RFP 适用: Discosoma WB, ELISA, IF, IP, IHC, FACS, IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Red Fluorescent Protein (RFP) antibodyRFP 适用: Discosoma WB, ELISA 宿主: 小鼠 Monoclonal 8E5-G7 unconjugated
anti-Red Fluorescent Protein (RFP) antibodyRFP 适用: Discosoma WB, ELISA 宿主: 小鸡 Polyclonal unconjugated
anti-Red Fluorescent Protein (RFP) antibodyRFP 适用: Discosoma WB, IF, IHC (fro), IHC (p) 宿主: 山羊 Polyclonal unconjugated
anti-Red Fluorescent Protein (RFP) antibody (Biotin)RFP 适用: Discosoma WB, ELISA, IF 宿主: 兔 Polyclonal Biotin
anti-Red Fluorescent Protein (RFP) antibody (Biotin)RFP 适用: Discosoma WB, ELISA 宿主: 小鸡 Polyclonal Biotin
anti-Red Fluorescent Protein (RFP) antibody (DyLight 633)RFP 适用: Discosoma WB, IF, IHC (fro) 宿主: 山羊 Polyclonal DyLight 633
anti-Red Fluorescent Protein (RFP) antibody (DyLight 550)RFP 适用: Discosoma WB, IF, IHC (fro) 宿主: 山羊 Polyclonal DyLight 550
anti-Red Fluorescent Protein (RFP) antibody (DyLight 488)RFP 适用: Discosoma WB, IF, IHC (fro) 宿主: 山羊 Polyclonal DyLight 488
anti-Red Fluorescent Protein (RFP) antibody (HRP)RFP 适用: Discosoma WB, ELISA, IF 宿主: 兔 Polyclonal HRP
-
- 应用备注
-
Polyclonal anti-RFP is designed to detect RFP and its variants. This antibody can be used todetect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotinconjugated polyclonal anti-RFP used in a sandwich ELISA with unconjugated anti-RFP iswell suited to titrate RFP in solution. The detection antibody conjugated to biotin issubsequently reacted with streptavidin conjugated HRP. Fluorochrome conjugatedpolyclonal anti-RFP can be used to detect RFP by immunofluorescence microscopy in cellexpression systems and can detect RFP containing inserts. Significant amplification ofsignal is achieved using fluorochrome conjugated polyclonal anti-RFP relative to thefluorescence of RFP alone. For immunoblotting use either alkaline phosphatase orperoxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins onwestern blots. Recommended Dilutions: ELISA: 1/10,000-1/50,000. Western blot: 1/2,000-1/10,000. Immunohistochemistry on Frozen Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 说明
-
Molar Ratio: 10-20 BAC molecules per IgG molecule
- 限制
- 仅限研究用
-
- 溶解方式
- Restore with 0.1 mL of deionized water (or equivalent).
- 浓度
- 1.0 mg/mL
- 缓冲液
- 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, 10 mg/mL BSA (IgG and Protease free)
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
-
- 抗原
- RFP (Red Fluorescent Protein (RFP))
- Abstract
- RFP 产品
- 背景
- Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. To utilize this feature, protein expression vectors are typically engineered with a nucleotide sequence that encodes the peptide epitope tag. The gene of interest is cloned in-frame relative to the tag and, upon expression, the protein of interest is synthesized as a fusion protein with the peptide tag. Fusion protein detection and/or purification is mediated by highly specific antibodies to the engineered peptide, thus eliminating the need for antibodies to proteins from each newly cloned gene. Commonly used epitope tags include glutathione-S-transferase (GST), c-myc, 6-histidine (6X-His), FLAG, green fluorescent protein (GFP), red fluorescent protein (RFP, DSRed), maltose binding protein (MBP), influenza A virus haemagglutinin (HA), b-galactosidase, and GAL4.Synonyms: DsRed Tag, Red fluorescent protein Tag
- 基因ID
- 86600
- UniProt
- Q9U6Y8
-
