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CHEK1 抗体 (pSer317)

Name: CHEK1 抗体 (pSer317)
SKU: ABIN389543
Availability: OutOfStock

This anti-CHEK1 antibody is a 兔多克隆 antibody detecting CHEK1 in WB, IHC (p) 和 DB. Suitable for 人. This Primary Antibody has been cited in 1 publication.

The anti-Phospho-CHK1- Pab (ABIN391324 and ABIN2841354) is used in Western blot for detection in, from left to right, Jurkat, , Hela, and HL60 tissue lysates. (CHEK1 抗体 (pSer317))

Dot blot analysis of anti-Phospho-CHK1- Antibody (ABIN389543 and ABIN2839588) on nitrocellulose membrane. (CHEK1 抗体 (pSer317))

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. (CHEK1 抗体 (pSer317))

IHC (p)

3 images

(1 reference)

产品编号 ABIN389543

发货至: 中国

Quick Overview for CHEK1 抗体 (pSer317) (ABIN389543)

抗原

See all CHEK1 抗体

CHEK1 (Checkpoint Kinase 1 (CHEK1))

适用

人

宿主

兔

克隆类型

多克隆

标记

This CHEK1 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Dot Blot (DB)

克隆位点

RB7845-RB18425

Discover our top product CHEK1 Primary Antibody

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pSer317

纯化方法

This antibody is purified through a protein A column, followed by peptide affinity purification.

免疫原

This CHK1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S317 of human CHK1.

亚型

Ig Fraction
anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibody
CHEK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Checkpoint Kinase 1 (CHEK1) (pSer301) antibody
CHEK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Checkpoint Kinase 1 (CHEK1) (pSer296) antibody
CHEK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Checkpoint Kinase 1 (CHEK1) (pSer286) antibody
CHEK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Checkpoint Kinase 1 (CHEK1) (pSer345) antibody
CHEK1 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

anti-Checkpoint Kinase 1 (CHEK1) (Middle Region) antibody
CHEK1 适用: 人, 小鼠, 大鼠, 犬, 兔, Cow, 马, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibody
CHEK1 适用: 小鼠, 小鸡 ELISA, IHC (p), ICC, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Checkpoint Kinase 1 (CHEK1) antibody
CHEK1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Monoclonal 2F2 unconjugated Recombinant Antibody

anti-Checkpoint Kinase 1 (CHEK1) antibody
CHEK1 适用: 人, 小鼠 WB, ELISA 宿主: 小鼠 Monoclonal 2G1D5 unconjugated

anti-Checkpoint Kinase 1 (CHEK1) (pSer296) antibody
CHEK1 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

应用备注

WB: 1:1000. IHC-P: 1:50~100. DB: 1:500

限制

仅限研究用
状态

Liquid

缓冲液

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

有效期

6 months
Awate, De Benedetti: "TLK1B mediated phosphorylation of Rad9 regulates its nuclear/cytoplasmic localization and cell cycle checkpoint." in: BMC molecular biology, Vol. 17, pp. 3, (2016) (PubMed).
抗原

CHEK1 (Checkpoint Kinase 1 (CHEK1))

别名

CHK1

背景

Checkpoint pathways control the order and timing of cell cycle transitions and ensure that critical events, such as DNA replication and chromosome segregation, are completed with high fidelity. The S. pombe Chk1 gene encodes a protein kinase that is required for the DNA damage checkpoint. Antibodies against CHK1 recognized a 54-kD protein on immunoblots of mammalian cell extracts. However,CHK1 is modified in response to DNA damage. In vitro, CHK1 directly phosphorylated a regulator of CDC2 tyrosine phosphorylation, CDC25C. In response to DNA damage, CHK1 phosphorylates and inhibits CDC25C, thus preventing activation of the CDC2-cyclin B complex and mitotic entry. CHK1 directly phosphorylates CDC25A during an unperturbed cell cycle, and that phosphorylation of CDC25A by CHK1 is required for cells to delay cell cycle progression in response to double-strand DNA breaks.

分子量

54434

基因ID

1111

NCBI登录号

NP_001107593, NP_001107594, NP_001231775, NP_001265

UniProt

O14757

途径

p53 Pathway, Apoptosis, Cell Division Cycle, DNA Damage Repair