Peptide ELISA: Limit dilution 1: 16000. Western blot: 1-3 μg/mL. Approx 37 kDa band observed in Human Brain lysates (calculatedMW of 37.7 kDa according to NP_149084.1, isoform delta). An additional faint 47 kDa bandconsistent with calculated MW of 40.7 kDa according to NP_149083.1 (isoform gamma) isalso observed. Immunohistochemistry: 1.0 μg/mL. In paraffin embedded Human Tonsil,stains a group of cells in the interfollicular area, below the epithelium of tonsillar criptaeand weak in germinal centre. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
0.5 mg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.