Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.1-0.3 μg/mL. Approx 57 kDa band observed in lysates of cell line A549. Intransfected HEK293 transiently expressing IRF5 a strong band of approx.67 kDa isobserved. This band is not observed in the non-transfected HEK293. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
0.5 mg/mL
缓冲液
Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.
Interferon regulatory factors (IRFs) are transcription factors that possess a novel helix turn helix DNA binding motif and mediate virus and interferon (IFN) induced signaling pathways. The IRF5 protein is structurally related to IRF6. Like other IRFs, IRF5 and IRF6 contain 5 conserved tryptophan residues in the 115 amino acid N terminal DNA binding domain. IRF5 has a central IRF association domain (IAD) and, like IRF7 and IRF8, is induced by IFNA/IFNB stimulation. IRF5 has a type I IFN transactivation potential similar to that of IRF1. IRF3 and IRF5 form heterodimers and are both required for effective induction of IFNA gene transcription.Synonyms: IRF-5, Interferon regulatory factor 5