Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
特异性
The antibody detects endogenous level of p56Dok-2 only when phosphorylated at tyrosine 299.
纯化方法
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
免疫原
Peptide sequence around phosphorylation site of pTyr299 (G-E-Y (p) -A-V) derived from Human p56Dok-2. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation Feng Cong, et,al. (1999) Mol. Cell. Biol , 19: 8314 - 8325. Serge Lemay, et,al. (2000) Mol. Cell. Biol , 20: 2743 - 2754. Ute Schaeper, et,al.(2000) J. Cell Biol , 149: 1419. Miyuki Honma, et,al. (2006) Genes Cells, 11: 143 - 151.