The antibody detects endogenous level of HER2 only when phosphorylated at tyrosine1221/tyrosine1222.
纯化方法
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
免疫原
Peptide sequence around phosphorylation site of pTyr1221, pTyr1222 (N-L-Y (p) -Y (p) -W) derived from Human HER2. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF-a and amphiregulin.