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Nestin 抗体 (AA 1464-1614)

This anti-Nestin antibody is a 小鼠 单克隆 antibody detecting Nestin in WB, IHC, ICC, IF, IHC (p) 和 IHC (fro). Suitable for 人.
产品编号 ABIN351499
发货至: 中国

Quick Overview for Nestin 抗体 (AA 1464-1614) (ABIN351499)

抗原

See all Nestin (NES) 抗体
Nestin (NES)

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This Nestin antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
  • 抗原表位

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    AA 1464-1614

    品牌

    IHC-plus™

    特异性

    This antibody is specific for human nestin. Species cross-reactivity: This antibody recognizes human nestin protein. It does not cross-react with rodent protein.

    无交叉反应

    Rodents

    纯化方法

    Protein G purified

    免疫原

    A fragment of the human nestin protein corresponding to residues 1464-1614.

    Type of Immunogen: Protein

    亚型

    IgG1 kappa
  • 应用备注

    Approved: ICC, IF, IHC, IHC-Fr, IHC-P (5 μg/mL), WB (1:1000 - 1:5000)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 1:1000-1:5000. Doublet band seen ~220-240kD.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS, 0.09 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term: 4°C
    Long term: -20°C.
  • 抗原

    Nestin (NES)

    别名

    Nestin

    背景

    Name/Gene ID: NES

    Synonyms: NES, Nestin, Nbla00170

    基因ID

    10763

    UniProt

    P48681
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