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- 抗原 See all p130 (RBL2) 抗体
- p130 (RBL2) (Retinoblastoma-Like 2 (p130) (RBL2))
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适用
- 人, Chimpanzee
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This p130 antibody is un-conjugated
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应用范围
- ELISA, Western Blotting (WB)
- 特异性
- This antiserum is directed against Spa310 and reacts with the Spa3120 domain of pRb2/p130 from human tissues. Based on the sequence we expect this antibody to react as well Spa310 from chimpanzee and orangutan, and with lesser affinity, Spa310 from horse, dog, bovine, rat and opossum.
- 交叉反应
- Chimpanzee
- 产品特性
- Spa310 is a 39 aa-long polypeptide encoded by a sequence which resides in the spacer region of the tumor suppressor Rb2 gene. Rb2 is a member of the retinoblastoma (Rb) gene family. Proteins in this family, which also include pRb/p105 and pRb/p107, are important cellular factors which play well-recognized roles as tumor and growth suppressors. Both p107 and pRb2/p130 share the ability to physically interact and inhibit the kinase activity of the Cdk2/Cyclin A and Cdk2/Cyclin E complexes, which play critical roles in cell cycle regulation. Spa310 is the region of the pRb2/p130 protein that is responsible for Cdk2/Cyclin E/A inhibition. Spa310 has also been shown to suppress cell growth as observed by colony formation, and to reduce volume of tumor growth in nude mice, likely through arrest in the G 0 /G 1 phase of the cell cycle. Understandably, the Spa310 small molecule represents a potentially significant pharmaceutical product in the treatment of hyperproliferative disorders.
- 纯化方法
- affinity purified
- 过滤
- Sterile filtered
- 免疫原
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This affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to the Spa310 sequence of pRb2/p130 protein. A residue of cysteine was added to facilitate coupling.
Immunogen Type: Peptide - 亚型
- IgG
- Top Product
- Discover our top product RBL2 Primary Antibody
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anti-Retinoblastoma-Like 2 (p130) (RBL2) (AA 939-1139) antibody
RBL2 适用: 人 ELISA, WB, FACS 宿主: 小鼠 Monoclonal 2C8A8 unconjugated
anti-Retinoblastoma-Like 2 (p130) (RBL2) (AA 165-194), (N-Term) antibodyRBL2 适用: 人 WB, FACS 宿主: 兔 Polyclonal RB31021 unconjugated
anti-Retinoblastoma-Like 2 (p130) (RBL2) (AA 878-913) antibodyRBL2 适用: 人, 大鼠 WB, IF, IHC, IHC (p), IP 宿主: 小鼠 Monoclonal 130P215 unconjugated
anti-Retinoblastoma-Like 2 (p130) (RBL2) (AA 100-200) antibodyRBL2 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Retinoblastoma-Like 2 (p130) (RBL2) (AA 417-616) antibodyRBL2 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Retinoblastoma-Like 2 (p130) (RBL2) antibodyRBL2 适用: 人, 大鼠, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Retinoblastoma-Like 2 (p130) (RBL2) antibodyRBL2 适用: 人, 大鼠, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Retinoblastoma-Like 2 (p130) (RBL2) (AA 416-515) antibodyRBL2 适用: 人 ELISA, WB 宿主: 小鼠 Polyclonal unconjugated
anti-Retinoblastoma-Like 2 (p130) (RBL2) (AA 416-515) antibodyRBL2 适用: 人 ELISA 宿主: 小鼠 Monoclonal 4D7 unconjugated
anti-Retinoblastoma-Like 2 (p130) (RBL2) (C-Term) antibodyRBL2 适用: 人, 大鼠 ELISA, WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- This affinity purified antibody is suitable for ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 130 kDa in size corresponding to pRb2/p130, and a band approximately 4.2kDa in size corresponding to Spa310 peptide (latter not shown), by western blotting in the appropriate cell lysate or extract.
- 说明
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Gene Name: RBL2
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.39 mg/mL
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is three (3) months from date of opening.
- 有效期
- 3 months
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- 抗原
- p130 (RBL2) (Retinoblastoma-Like 2 (p130) (RBL2))
- 别名
- pRb2 p130 (RBL2 产品)
- 别名
- RBL2 antibody, RBR2 antibody, RRB2a antibody, PRB2 antibody, RBR-2 antibody, Rb2 antibody, p130 antibody, P130 antibody, retinoblastoma-like 2 (p130) antibody, RB transcriptional corepressor like 2 antibody, related to retinoblastoma 2 antibody, RBL2 antibody, LOC541652 antibody, Rbl2 antibody
- 背景
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Spa310 is a 39 aa-long polypeptide encoded by a sequence which resides in the spacer region of the tumor suppressor Rb2 gene. Rb2 is a member of the retinoblastoma (Rb) gene family. Proteins in this family, which also include pRb/p105 and pRb/p107, are important cellular factors which play well-recognized roles as tumor and growth suppressors. Both p107 and pRb2/p130 share the ability to physically interact and inhibit the kinase activity of the Cdk2/Cyclin A and Cdk2/Cyclin E complexes, which play critical roles in cell cycle regulation. Spa310 is the region of the pRb2/p130 protein that is responsible for Cdk2/Cyclin E/A inhibition. Spa310 has also been shown to suppress cell growth as observed by colony formation, and to reduce volume of tumor growth in nude mice, likely through arrest in the G 0 /G 1 phase of the cell cycle. Understandably, the Spa310 small molecule represents a potentially significant pharmaceutical product in the treatment of hyperproliferative disorders.
Synonyms: 130 kDa retinoblastoma associated protein antibody, RBR2 antibody, Retinoblastoma like 2 antibody, Retinoblastoma like protein 2 antibody, Retinoblastoma Related Gene antibody - 基因ID
- 5934
- UniProt
- Q08999
- 途径
- Cell Division Cycle, Mitotic G1-G1/S Phases
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