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SIRT1 抗体 (AA 244-498)

Name: SIRT1 抗体 (AA 244-498)
SKU: ABIN1860561
Availability: OutOfStock

SIRT1 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C4 unconjugated

Western Blotting (WB) image for anti-Sirtuin 1 (SIRT1) (AA 244-498) antibody (ABIN1860561) (SIRT1 抗体 (AA 244-498))

1 image

(1 reference)

产品编号 ABIN1860561

发货至: 中国

Datasheet as PDF

抗原 See all SIRT1 抗体

SIRT1 (Sirtuin 1 (SIRT1))
抗原表位
27

16

16

9

8

7

6

6

5

4

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 244-498
适用
142

88

57

34

5

3

2

2

2

2

2

1

1
人
宿主
128

20

2

2

1
小鼠
Compatible Secondaries
克隆类型
112

41
单克隆
标记
74

14

10

5

5

5

5

5

5

4

4

3

3

3

3

3

1

1
This SIRT1 antibody is un-conjugated
应用范围
125

50

50

41

39

35

26

23

20

14

9

4

4

4

2

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

原理

Monoclonal Antibody to Sirtuin 1 (SIRT1)

序列

MGHHHHHHSG SEF-EDAVKLL QECKKIIVLT GAGVSVSCGI PDFRSRDGIY ARLAVDFPDL PDPQAMFDIE YFRKDPRPFF KFAKEIYPGQ FQPSLCHKFI ALSDKEGKLL RNYTQNIDTL EQVAGIQRII QCHGSFATAS CLICKYKVDC EAVRGDIFNQ VVPRCPRCPA DEPLAIMKPE IVFFGENLPE QFHRAMKYDK DEVDLLIVIG SSLKVRPVAL IPSSIPHEVP QILINREPLP HLHFDVELLG DCDVIINELC HRLGGEYA

特异性

The antibody is a mouse monoclonal antibody raised against SIRT1. It has been selected for its ability to recognize SIRT1 in immunohistochemical staining and western blotting.

交叉反应

小鼠, 大鼠

纯化方法

Protein A + Protein G affinity chromatography

免疫原

Recombinant Sirtuin 1 (SIRT1) corresdonding to Glu244~Ala498 with N-terminal His Tag

克隆位点

C4

亚型

IgG2b kappa

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anti-Sirtuin 1 (SIRT1) (AA 566-601), (C-Term) antibody
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KO Validated SIRT1 适用: 人 WB, IHC, IP, IF, ChIP 宿主: 兔 Polyclonal unconjugated

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SIRT1 适用: 人, 小鼠, 大鼠, 犬, 兔, Cow, 斑马鱼, 豚鼠, 马 WB 宿主: 兔 Polyclonal unconjugated

anti-Sirtuin 1 (SIRT1) (AA 566-601) antibody
SIRT1 适用: 人, 小鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Sirtuin 1 (SIRT1) (AA 456-555) antibody
SIRT1 适用: 人 WB, IF, IHC (p), EIA 宿主: 小鼠 Monoclonal 7B7 unconjugated

anti-Sirtuin 1 (SIRT1) (pSer47) antibody
SIRT1 适用: 人, 小鼠, 大鼠, 犬 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

应用备注

Western blotting: 0.5-5 μg/mL Immunocytochemistry in formalin fixed cells: 5-30 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-30 μg/mL Immunohistochemistry in paraffin section: 5-30 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

说明

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

限制

仅限研究用
状态

Liquid

浓度

Lot specific

缓冲液

PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

储存液

Sodium azide

注意事项

WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

注意事项

Avoid repeated freeze/thaw cycles

储存条件

4 °C,-20 °C

储存方法

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

有效期

24 months
Zbroch, Bazyluk, Malyszko, Koc-Zorawska, Rydzewska-Rosolowska, Kakareko, Hryszko: "The Serum Concentration of Anti-Aging Proteins, Sirtuin1 and αKlotho in Patients with End-Stage Kidney Disease on Maintenance Hemodialysis." in: Clinical interventions in aging, Vol. 15, pp. 387-393, (2020) (PubMed).
抗原

SIRT1 (Sirtuin 1 (SIRT1))

别名

Sirtuin 1 (SIRT1 产品)

别名

sirtuin 1 antibody, AA673258 antibody, SIR2L1 antibody, Sir2 antibody, Sir2a antibody, Sir2alpha antibody, sir2l1 antibody, sirtuin antibody, SIR2 antibody, sirtuin 1 antibody, sirtuin 1 L homeolog antibody, SIRT1 antibody, SRT1 antibody, Sirt1 antibody, sirt1.L antibody

背景

SIR2L1, Silent Mating Type Information Regulation 2 Homolog 1, NAD-Dependent Deacetylase Sirtuin-1, SIR2-like protein 1, SirtT1 75 kDa fragment

途径

MAPK Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling