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EGF 抗体 (AA 45-275)

Name: EGF 抗体 (AA 45-275)
SKU: ABIN3201444
Availability: OutOfStock

EGF 适用: Pig WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal 2# unconjugated

Western Blotting (WB) image for anti-Epidermal Growth Factor (EGF) (AA 45-275) antibody (ABIN3201444) (EGF 抗体 (AA 45-275))

1 image

产品编号 ABIN3201444

发货至: 中国

Datasheet as PDF

抗原 See all EGF 抗体

EGF (Epidermal Growth Factor (EGF))
抗原表位
45

16

10

8

7

7

7

7

6

6

6

5

5

4

4

4

4

3

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 45-275
适用
143

85

50

12

11

5

4

1

1

1
Pig
宿主
209

29

9

3

1

1
小鼠
Compatible Secondaries
克隆类型
215

36
单克隆
标记
112

36

19

14

7

7

5

5

5

5

5

5

4

4

4

4

4

2

1

1

1

1

1
This EGF antibody is un-conjugated
应用范围
199

86

62

53

53

29

25

24

16

16

15

14

8

8

4

4

3

2

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

原理

Monoclonal Antibody to Epidermal Growth Factor (EGF)

序列

MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- APFLIF SHGNSIFRID LEGTNHEQLV ADAGISVLMD FHYNEERIYW VDLERQLLQR VFLNGTRQEK VCNLEKNVSG MAINWINEEL IWSNQQEGTI TVTDMKGNNS RVLLSALKYP ANVAVDPVER LMFWSSVVAG SLHRADVTGV EVRLLLETSE EIAAVSLDVL DKRLFWIQYN REGGSSRICS CDYDGGSVHF SKHLTQHNVF AMSLFGDHIF YSTWKKKTIW VANKH

特异性

The antibody is a mouse monoclonal antibody raised against EGF. It has been selected for its ability to recognize EGF in immunohistochemical staining and western blotting.

纯化方法

Protein A + Protein G affinity chromatography

免疫原

Recombinant Epidermal Growth Factor (EGF) corresdonding to Ala45~His275 with N-terminal His Tag

克隆位点

2#

亚型

IgG2b kappa

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应用备注

Western blotting: 0.5-5 μg/mL Immunohistochemistry: 5-30 μg/mL Immunocytochemistry: 5-30 μg/mL Optimal working dilutions must be determined by end user.

说明

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

限制

仅限研究用
状态

Liquid

浓度

Lot specific

缓冲液

0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

储存液

ProClin

注意事项

WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

注意事项

Avoid repeated freeze/thaw cycles

储存条件

4 °C,-20 °C

储存方法

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

有效期

24 months
抗原

EGF (Epidermal Growth Factor (EGF))

别名

Epidermal Growth Factor (EGF 产品)

别名

HOMG4 antibody, URG antibody, AI790464 antibody, CEGF antibody, epidermal growth factor antibody, pro-epidermal growth factor antibody, EGF antibody, egf antibody, CpipJ_CPIJ020278 antibody, Egf antibody

背景

URG, Beta-Urogastrone, Pro-epidermal growth factor

途径

NF-kappaB Signaling, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation