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HBEGF 抗体 (Bound, EGF Like Domain, Soluble)

HBEGF 适用: 人 WB, ELISA, IP, ICC, IF 宿主: 小鼠 Monoclonal 4G10 unconjugated
产品编号 ABIN3201018
发货至: 中国
  • 抗原 See all HBEGF 抗体
    HBEGF (Heparin-Binding EGF-Like Growth Factor (HBEGF))
    抗原表位
    • 15
    • 15
    • 14
    • 8
    • 7
    • 7
    • 6
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Bound, EGF Like Domain, Soluble
    适用
    • 76
    • 20
    • 19
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    宿主
    • 70
    • 9
    小鼠
    克隆类型
    • 67
    • 11
    • 1
    单克隆
    标记
    • 27
    • 10
    • 7
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    This HBEGF antibody is un-conjugated
    应用范围
    • 35
    • 28
    • 26
    • 15
    • 13
    • 10
    • 7
    • 7
    • 6
    • 6
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)
    交叉反应 (详细)
    Does not react with mouse.
    过滤
    Sterile filtered
    免疫原
    RecombinanthumanHB-EGFectodomainexpressed inSF21 cell
    克隆位点
    4G10
    亚型
    IgG1
    Top Product
    Discover our top product HBEGF Primary Antibody
  • 应用备注
    The antibody can react with both soluble and cell-surface attached forms of HG-EGF.
    1) Westernblotting: 0. 2~1 g/mL
    2) Immunoprecipitation: 1 g/mL
    3) Indirect immuno-fluorescence staining: 5~11 g/mL
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1 mg/mL
    缓冲液
    PBS with 50 % Glycerol
    储存液
    Azide free
    储存条件
    -20 °C
    储存方法
    Upon arrival centrifuge briefly, aliquote and store at -20 C.
  • Miyamoto, Hirata, Yamazaki, Kageyama, Hasuwa, Mizushima, Tanaka, Yagi, Sonoda, Kai, Kanoh, Nakano, Mekada: "Heparin-binding EGF-like growth factor is a promising target for ovarian cancer therapy." in: Cancer research, Vol. 64, Issue 16, pp. 5720-7, (2004) (PubMed).

    Prenzel, Zwick, Daub, Leserer, Abraham, Wallasch, Ullrich: "EGF receptor transactivation by G-protein-coupled receptors requires metalloproteinase cleavage of proHB-EGF." in: Nature, Vol. 402, Issue 6764, pp. 884-8, (2000) (PubMed).

    Iwamoto, Higashiyama, Mitamura, Taniguchi, Klagsbrun, Mekada et al.: "Heparin-binding EGF-like growth factor, which acts as the diphtheria toxin receptor, forms a complex with membrane protein DRAP27/CD9, which up-regulates functional receptors and diphtheria toxin ..." in: The EMBO journal, Vol. 13, Issue 10, pp. 2322-30, (1994) (PubMed).

    Higashiyama, Abraham, Miller, Fiddes, Klagsbrun: "A heparin-binding growth factor secreted by macrophage-like cells that is related to EGF." in: Science (New York, N.Y.), Vol. 251, Issue 4996, pp. 936-9, (1991) (PubMed).

  • 抗原
    HBEGF (Heparin-Binding EGF-Like Growth Factor (HBEGF))
    别名
    HB-EGF (HBEGF 产品)
    别名
    hbegf antibody, zgc:165443 antibody, HBEGF antibody, zgc:175091 antibody, DTR antibody, DTS antibody, DTSF antibody, HEGFL antibody, Dtr antibody, GFHB antibody, Hb-egf antibody, Hegfl antibody, AW047313 antibody, Dts antibody, HB-EGF antibody, heparin-binding EGF-like growth factor b antibody, heparin binding EGF like growth factor antibody, Heparin-binding EGF-like growth factor antibody, heparin-binding EGF-like growth factor a antibody, heparin-binding EGF-like growth factor antibody, hbegfb antibody, HBEGF antibody, hbegf antibody, hbegfa antibody, Hbegf antibody
    背景
    Background: Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is synthesized as a membrane-anchored precursor that is proteolytically cleaved to release the soluble mature growth factor, HB-EGF. The former functions as juxtacrine and the latter as paracrine growth factor. Soluble HB-EGF shows several forms in Western blotting with apparent molecular weights 19~27 kDa due to heterogeneous O-glycosylation and N-terminal truncation. HB-EGF activates EGFR and ErbB4 and promotes the development in many tissues. In human ProHB-EGF is the cellular receptor for diphtheria toxin. Non-toxic mutant of diphtheria toxin, CRM197, inhibits HB-EGF function. As HB-EGF level is elevated in most ovarian cancer, CRM197 is being tested as an anticancer drug. The hybridoma clone 4G10 was established and characterized in the laboratoryof Prof. E.Mekadaof OsakaUniversity, who is a leadingscientist inthis field.
    UniProt
    Q99075
    途径
    RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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