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POU2AF1 抗体 (C-Term)

POU2AF1 适用: 大鼠, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN3044424
发货至: 中国
  • 抗原 See all POU2AF1 抗体
    POU2AF1 (POU Class 2 Associating Factor 1 (POU2AF1))
    抗原表位
    • 15
    • 15
    • 14
    • 8
    • 6
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    AA 238-256, C-Term
    适用
    • 60
    • 19
    • 5
    大鼠, 小鼠
    宿主
    • 65
    • 9
    • 2
    克隆类型
    • 63
    • 13
    多克隆
    标记
    • 34
    • 6
    • 5
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This POU2AF1 antibody is un-conjugated
    应用范围
    • 51
    • 32
    • 26
    • 26
    • 24
    • 19
    • 14
    • 6
    • 6
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    原理
    Rabbit IgG polyclonal antibody for POU domain class 2-associating factor 1(POU2AF1) detection. Tested with WB, IHC-P in Mouse,Rat.
    序列
    EEEESNTYEL NHTLSVEGF
    交叉反应 (详细)
    No cross reactivity with other proteins.
    产品特性
    Rabbit IgG polyclonal antibody for POU domain class 2-associating factor 1(POU2AF1) detection. Tested with WB, IHC-P in Mouse,Rat.
    Gene Name: POU class 2 associating factor 1
    Protein Name: POU domain class 2-associating factor 1
    纯化方法
    Immunogen affinity purified.
    免疫原
    A synthetic peptide corresponding to a sequence at the C-terminus of mouse BOB1(238-256aa EEEESNTYELNHTLSVEGF), different from the related rat sequence by one amino acid, and from human sequence by four amino acids.
    亚型
    IgG
    Top Product
    Discover our top product POU2AF1 Primary Antibody
  • 应用备注
    WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse
    IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
    Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
    说明

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    浓度
    500 μg/mL
    缓冲液
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
    储存液
    Thimerosal (Merthiolate), Sodium azide
    注意事项
    This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C/-20 °C
    储存方法
    At -20°C for one year. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
    有效期
    12 months
  • 抗原
    POU2AF1 (POU Class 2 Associating Factor 1 (POU2AF1))
    别名
    POU2AF1 (POU2AF1 产品)
    别名
    BOB1 antibody, OBF-1 antibody, OBF1 antibody, OCAB antibody, BOB-1 antibody, BOB.1 antibody, Bob-1 antibody, Bob1 antibody, OBF.1 antibody, OCA-B antibody, POU class 2 associating factor 1 antibody, POU domain, class 2, associating factor 1 antibody, POU2AF1 antibody, Pou2af1 antibody
    背景
    POU2AF1(POU domain class 2-associating factor 1), also known as OBF1, BOB1 and OCAB, is a protein that in humans is encoded by the POU2AF1 gene. POU domain proteins contain a bipartite DNA-binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA. The versatility of POU protein operation is additionally conferred at the dimerization level. Casellas et al.(2002) reported that OCAB is essential for V(D)J recombination of a subset of Ig-kappa genes. They showed that OCAB modulates recombination by directly enhancing Ig-kappa gene transcription in vivo. Siegel et al.(2006) suggested that, together with dysregulation of other OCAB target genes, altered regulation of SYK may help explain the magnitude of defects observed in B-cell development, including the pre-B1-to-pre-B2 transition, and immune responses in Ocab -/- mice.

    Synonyms: B cell Oct binding protein 1 antibody|B cell specific coactivator OBF 1 antibody|B cell specific coactivator OBF1 antibody|B-cell-specific coactivator OBF-1 antibody|BOB 1 antibody|BOB-1 antibody|OBF 1 antibody|OBF1 antibody|OBF1_HUMAN antibody|OCA B antibody|OCA-B antibody|OCAB antibody|OCT binding factor 1 antibody|OCT-binding factor 1 antibody|POU domain class 2 associating factor 1 antibody|POU domain class 2-associating factor 1 antibody|POU2AF1 antibody
    UniProt
    Q64693
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