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MCM2 抗体 (C-Term)

MCM2 适用: 小鼠, 大鼠 WB, IHC, IF, FACS, ICC 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN3044274
发货至: 中国
  • 抗原 See all MCM2 抗体
    MCM2 (Minichromosome Maintenance Complex Component 2 (MCM2))
    抗原表位
    • 19
    • 15
    • 9
    • 8
    • 8
    • 7
    • 7
    • 6
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 884-904, C-Term
    适用
    • 123
    • 69
    • 28
    • 9
    • 9
    • 8
    • 7
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    小鼠, 大鼠
    宿主
    • 120
    • 13
    • 2
    • 1
    克隆类型
    • 112
    • 24
    多克隆
    标记
    • 79
    • 7
    • 6
    • 6
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    This MCM2 antibody is un-conjugated
    应用范围
    • 82
    • 51
    • 42
    • 28
    • 27
    • 26
    • 25
    • 24
    • 18
    • 13
    • 6
    • 4
    • 3
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)
    原理
    Anti-MCM2 Antibody Picoband®
    序列
    DLFKFNKFSR DLKRKLILQQ F
    交叉反应 (详细)
    No cross-reactivity with other proteins
    产品特性
    Anti-MCM2 Antibody (ABIN3044274). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
    纯化方法
    Immunogen affinity purified.
    免疫原
    A synthetic peptide corresponding to a sequence at the C-terminus of mouse MCM2, different from the related rat sequence by one amino acid.
    亚型
    IgG
    Top Product
    Discover our top product MCM2 Primary Antibody
  • 应用备注
    Western blot, 0.1-0.5 μg/mL, Mouse, Rat
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat
    Immunocytochemistry , 0.5-1 μg/mL, Mouse, Rat
    Immunocytochemistry/Immunofluorescence, 5 μg/mL, Mouse
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Mouse
    Blow, J. J., Laskey, R. A. A role for the nuclear envelope in controlling DNA replication within the cell cycle. Nature 332: 546-548, 1988. 2. Burkhart, R., Schulte, D., Hu, D., Musahl, C., Gohring, F., Knippers, R. Interactions of human nuclear proteins P1Mcm3 and P1Cdc46. Europ. J. Biochem. 228: 431-438, 1995. 3. Todorov IT, Lavigne J, Sakr F, Kaneva R, Foisy S, Bibor-Hardy V (Jul 1991). "Nuclear matrix protein mitotin messenger RNA is expressed at constant levels during the cell cycle". Biochem Biophys Res Commun 177 (1): 395-400.
    说明

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    浓度
    500 μg/mL
    缓冲液
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
    储存液
    Thimerosal (Merthiolate), Sodium azide
    注意事项
    This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C,-20 °C
    储存方法
    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
    有效期
    12 months
  • 抗原
    MCM2 (Minichromosome Maintenance Complex Component 2 (MCM2))
    别名
    MCM2 (MCM2 产品)
    背景

    Synonyms: DNA replication licensing factor MCM2,3.6.4.12,Minichromosome maintenance protein 2 homolog,Nuclear protein BM28,Mcm2,Bm28, Cdcl1, Kiaa0030, Mcmd2,

    Background: MCM2 (MINICHROMOSOME MAINTENANCE, S. CEREVISIAE, HOMOLOG OF, 2),also known as MITOTIN, CDCL1 or BM28, is a human nuclear protein that plays an important role in 2 crucial steps of the cell cycle, namely, onset of DNA replication and cell division. And it is similar to members of the family of early S-phase proteins. The MCM2 gene is mapped to 3q21.3. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. In the G0 stage, the MCM2 and MCM5 proteins were much less abundant than the MCM7 and MCM3 proteins, which suggests that the MCM proteins are not present in stoichiometric amounts and that only a proportion of these molecules actively participate in cell cycle regulation as part of MCM complexes.

    Sequence Similarities: Belongs to the MCM family.

    分子量
    102 kDa
    UniProt
    P97310
    途径
    DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA
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