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- 抗原 See all RFC1 抗体
- RFC1 (Replication Factor C (Activator 1) 1, 145kDa (RFC1))
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抗原表位
- AA 46-64, N-Term
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This RFC1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 原理
- Rabbit IgG polyclonal antibody for Replication factor C subunit 1(RFC1) detection. Tested with WB, IHC-P in Human.
- 序列
- NSSRKEDDFK QKQPSKKKR
- 交叉反应 （详细）
- No cross reactivity with other proteins.
- 产品特性
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Rabbit IgG polyclonal antibody for Replication factor C subunit 1(RFC1) detection. Tested with WB, IHC-P in Human.
Gene Name: replication factor C(activator 1) 1, 145 kDa
Protein Name: Replication factor C subunit 1 - 纯化方法
- Immunogen affinity purified.
- 免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of human RFC1(46-64aa NSSRKEDDFKQKQPSKKKR).
- 亚型
- IgG
- Top Product
- Discover our top product RFC1 Primary Antibody
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anti-Replication Factor C (Activator 1) 1, 145kDa (RFC1) (C-Term) antibody
RFC1 适用: 人 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Replication Factor C (Activator 1) 1, 145kDa (RFC1) (AA 400-700) antibodyRFC1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Replication Factor C (Activator 1) 1, 145kDa (RFC1) (AA 1-110) antibodyRFC1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3F8 unconjugated
anti-Replication Factor C (Activator 1) 1, 145kDa (RFC1) (Internal Region) antibodyRFC1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Replication Factor C (Activator 1) 1, 145kDa (RFC1) (AA 401-500) antibodyRFC1 适用: 人, 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro), ICC 宿主: 兔 Polyclonal unconjugated
anti-Replication Factor C (Activator 1) 1, 145kDa (RFC1) (C-Term) antibodyRFC1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Replication Factor C (Activator 1) 1, 145kDa (RFC1) (AA 1-110) antibodyRFC1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Replication Factor C (Activator 1) 1, 145kDa (RFC1) (Internal Region) antibodyRFC1 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Replication Factor C (Activator 1) 1, 145kDa (RFC1) (AA 1-110) antibodyRFC1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3F8 unconjugated
anti-Replication Factor C (Activator 1) 1, 145kDa (RFC1) (AA 935-984) antibodyRFC1 适用: 人, 小鼠, 豚鼠, 兔, 犬, 马, 猴 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. - 说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
- 储存液
- Thimerosal (Merthiolate), Sodium azide
- 注意事项
- This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing. - 有效期
- 12 months
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- 抗原
- RFC1 (Replication Factor C (Activator 1) 1, 145kDa (RFC1))
- 别名
- RFC1 (RFC1 产品)
- 别名
- A1 antibody, MHCBFB antibody, PO-GA antibody, RECC1 antibody, RFC antibody, RFC140 antibody, AtRFC1 antibody, replication factor C 1 antibody, replication factor C1 antibody, 140kDa antibody, Alp145 antibody, Recc1 antibody, Replication factor C1 antibody, si:dkey-170e5.1 antibody, zgc:162875 antibody, replication factor C subunit 1 antibody, replication factor C1 antibody, replication factor C (activator 1) 1 antibody, DNA replication factor C complex subunit Rfc1 antibody, RFC1 antibody, Rfc1 antibody, rfc1 antibody
- 背景
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RFC(Replication Factor C), also called activator-1, is a multimeric primer-recognition protein consisting of 5 distinct subunits of 145, 40, 38, 37, and 36.5 kD. Human RFC was purified from extracts of HeLa cells as a host factor essential for the in vitro replication of simian virus 40(SV40) DNA. RFC, in the presence of ATP, assembles proliferating-cell nuclear antigen and DNA polymerase-delta or polymerase-epsilon on primed DNA templates. Tight interfacial coordination of the ATP analog ATP-gamma-S by RFC resulted in a spiral arrangement of the ATPase domains of the clamp loader above the PCNA ring.
Synonyms: A1 140 kDa subunit antibody|A1 antibody|A1 P145 Activator 1 large subunit antibody|Activator 1 140 kDa subunit antibody|Activator 1 large subunit antibody|Activator 1 subunit 1 antibody|DNA binding Protein PO GA antibody|DNA-binding protein PO-GA antibody|MHC binding factor beta antibody|MHCBFB antibody|PO GA antibody|RECC1 antibody|Replication factor C(activator 1) 1, 145 kDa antibody|Replication factor C 140 kDa subunit antibody|Replication factor C antibody|Replication factor C large subunit antibody|Replication factor C subunit 1 antibody|Replication factor C1 antibody|RF-C 140 kDa subunit antibody|RFC antibody|RFC1 antibody|RFC1_HUMAN antibody|RFC140 antibody|RFC140 Replication Factor C 140 kDa subunit antibody - UniProt
- P35251
- 途径
- Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA, Dicarboxylic Acid Transport
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