Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
纯化方法
Antigen affinity
免疫原
An amino acid sequence from the middle region of human Connexin 32/GJB1 (RACARRAQRRSNPPSRK) was used as the immunogen for this Connexin 32 antibody (100% homologous in human, mouse and rat).
The stated application concentrations are suggested starting amounts. Titration of the Connexin 32 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL
限制
仅限研究用
缓冲液
0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
储存条件
-20 °C
储存方法
After reconstitution, the Connexin 32 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Connexins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells(Bergoffen et al., 1993). For a general discussion of connexin proteins, also known as Gap junction beta-1 protein. Gap junction beta-1 protein is a protein that in humans is encoded by the GJB1 gene. In melanocytic cells GJB1 gene expression may be regulated by MITF.