Keratin Acidic (AE1) 抗体
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- 抗原 See all Keratin Acidic (AE1) 抗体
- Keratin Acidic (AE1)
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适用
- 人, 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- An amino acid sequence common to acidic/type I keratins was used as the immunogen for the Acidic Cytokeratin antibody.
- 克隆位点
- ACCK1-1
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product AE1 Primary Antibody
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anti-Keratin Acidic (AE1) antibody
AE1 适用: 人 IHC, WB 宿主: 大鼠 Monoclonal OTI1D10 unconjugated
anti-Keratin Acidic (AE1) antibodyAE1 适用: 人, 大鼠, 小鸡, 小鼠, 兔, 犬, Cat, Non-Human Primate WB, FACS, IHC (p), ICC, IHC (fro) 宿主: 小鼠 Monoclonal AE1 unconjugated
anti-Keratin Acidic (AE1) antibodyAE1 适用: 人, 大鼠, 小鸡, 小鼠, 兔, 猴, Cow, Pig IHC (p), ICC, IHC (fro) 宿主: 小鼠 Monoclonal AE1 unconjugated
anti-Keratin Acidic (AE1) antibody (PE)AE1 适用: 人, 大鼠, 小鸡, 小鼠, 兔, 犬, Cat, Non-Human Primate FACS 宿主: 小鼠 Monoclonal AE1 PE
anti-Keratin Acidic (AE1) antibodyAE1 适用: 人, 大鼠, 小鸡, 小鼠, 兔, 猴, Cow, 犬, Turtle IF, WB, FACS, IHC (p) 宿主: 小鼠 Monoclonal AE1 unconjugated
anti-Keratin Acidic (AE1) antibodyAE1 适用: 人, 大鼠 IF, WB, FACS, IHC (p) 宿主: 小鼠 Monoclonal KRTL-1077 unconjugated
anti-Keratin Acidic (AE1) antibody (PE)AE1 适用: 人, 大鼠, 小鸡, 小鼠, 兔, 猴, 犬, Cat IHC, WB, FACS, IF/ICC 宿主: 小鼠 Monoclonal PE
anti-Keratin Acidic (AE1) antibodyAE1 适用: 人 IF, IHC (p) 宿主: 兔 Monoclonal RMAK1-1 unconjugated Recombinant Antibody
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- 应用备注
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Titering of the Acidic Cytokeratin antibody may be required for optimal performance.
1. FFPE testing requires sections to be boiled in pH 6 10 mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
3. This antibody will detect the following cytokeratins (CK): CK10: 56 kDa, CK14: 50 kDa, CK15: 50 kDa, CK16: 48 kDa, CK19: 40 kDa.\. Western blot: 1-2 μg/mL (3),Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2) - 限制
- 仅限研究用
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- 浓度
- 0.2 mg/mL
- 缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store the Acidic Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- 抗原
- Keratin Acidic (AE1)
- 别名
- Acidic Cytokeratin (AE1 产品)
- 背景
- There are two types of cytokeratins/keratins/CKs: the acidic type I cytokeratins and the basic or neutral type II cytokeratins. The subsets of cytokeratins which an epithelial cell expresses depends mainly on the type of epithelium, the moment in the course of terminal differentiation and the stage of development. Thus this specific keratin fingerprint allows the classification of all epithelia upon their keratin expression profile. Furthermore this applies also to the malignant counterparts of the epithelia (carcinomas), as the keratin profile tends to remain constant when an epithelium undergoes malignant transformation. The main clinical implication is that the study of the keratin profile by immunohistochemistry techniques is a tool of immense value widely used for tumor diagnosis and characterization in surgical pathology. [Wiki]
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