Keratin Acidic (AE1) 抗体
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北京 101111
Quick Overview for Keratin Acidic (AE1) 抗体 (ABIN3023883)
抗原
See all Keratin Acidic (AE1) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
克隆位点
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原理
- Acidic Cytokeratin Antibody (azide and preservative free)
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产品特性
- This mAb recognizes the 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40 kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
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纯化方法
- Protein G affinity chromatography
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免疫原
- Recombinant human KRT77 protein was used as the immunogen for the Acidic Cytokeratin antibody.
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亚型
- IgG1, kappa
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anti-Keratin Acidic (AE1) antibody
AE1 适用: 人 IHC, WB 宿主: 大鼠 Monoclonal OTI1D10 unconjugated
anti-Keratin Acidic (AE1) antibodyAE1 适用: 人, 大鼠, 小鼠, 小鸡, Cow, 兔, 猴, Pig IHC (p), ICC, IHC (fro) 宿主: 小鼠 Monoclonal AE1 unconjugated
anti-Keratin Acidic (AE1) antibodyCarrier-free AE1 适用: 人, 大鼠, 小鼠 WB, IF, IHC (p), FACS 宿主: 小鼠 Monoclonal AE1 unconjugated
anti-Keratin Acidic (AE1) antibodyAE1 适用: 人 IHC (p) 宿主: 兔 Monoclonal RMAK1-1 unconjugated Recombinant Antibody
anti-Keratin Acidic (AE1) antibodyCarrier-free AE1 适用: 人 IHC (p) 宿主: 兔 Monoclonal RMAK1-1 unconjugated Recombinant Antibody
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应用备注
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Optimal dilution of the Acidic Cytokeratin antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the Acidic Cytokeratin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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- Keratin Acidic (AE1)
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别名
- Acidic Cytokeratin
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背景
- This mAb recognizes the 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40 kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
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UniProt
- Q7Z794
抗原
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