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- 抗原 See all MCAM 抗体
- MCAM (Melanoma Cell Adhesion Molecule (MCAM))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This MCAM antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- Recombinant human protein was used as the immunogen for the CD146 antibody.
- 克隆位点
- MUC18-1130
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product MCAM Primary Antibody
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anti-Melanoma Cell Adhesion Molecule (MCAM) (AA 84-189) antibody
MCAM 适用: 人 FACS, IHC, WB, ELISA 宿主: 小鼠 Monoclonal 6C3E6 unconjugated
anti-Melanoma Cell Adhesion Molecule (MCAM) (AA 50-646) antibodyMCAM 适用: 人 FACS, WB, IF, ELISA 宿主: 小鼠 Monoclonal 4D10C7 unconjugated
anti-Melanoma Cell Adhesion Molecule (MCAM) (AA 447-646) antibodyMCAM 适用: 人 IHC, WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Melanoma Cell Adhesion Molecule (MCAM) antibodyMCAM 适用: 人 FACS, IHC, WB, IF, ELISA 宿主: 兔 Monoclonal 8A10 unconjugated Recombinant Antibody
anti-Melanoma Cell Adhesion Molecule (MCAM) antibodyMCAM 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Melanoma Cell Adhesion Molecule (MCAM) (AA 226-374) antibodyMCAM 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal MCAM-3046 unconjugated
anti-Melanoma Cell Adhesion Molecule (MCAM) (AA 1-646) antibodyMCAM 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Melanoma Cell Adhesion Molecule (MCAM) (AA 201-300) antibodyMCAM 适用: 人, 大鼠 FACS, WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Melanoma Cell Adhesion Molecule (MCAM) antibodyMCAM 适用: 人, 小鼠, 大鼠 IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Melanoma Cell Adhesion Molecule (MCAM) antibodyMCAM 适用: 人 IHC, WB, ELISA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Optimal dilution of the CD146 antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2) - 限制
- 仅限研究用
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- 浓度
- 0.2 mg/mL
- 缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store the CD146 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- 抗原
- MCAM (Melanoma Cell Adhesion Molecule (MCAM))
- 别名
- CD146 (MCAM 产品)
- 别名
- CD146 antibody, MUC18 antibody, Muc18 antibody, 1-gicerin antibody, AV025631 antibody, CD149 antibody, s-endo antibody, s-gicerin antibody, cd146 antibody, gicerin antibody, id:ibd3512 antibody, mcam antibody, MCAM antibody, melanoma cell adhesion molecule antibody, melanoma cell adhesion molecule b antibody, MCAM antibody, Mcam antibody, mcamb antibody
- 背景
- The human Mel-CAM gene maps to chromosome 11q23 and encodes a trans-membrane glycoprotein, also designated MCAM, MUC 18 or CD146, that belongs to the immunoglobulin superfamily and functions as a Ca2+-independent cell adhesion molecule. CD146 expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes. It is found on 80 % of advanced primary human melanomas and correlates well with development of metastatic disease.
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