TGFA 抗体 (C-Term)
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- 抗原 See all TGFA 抗体
- TGFA (Transforming Growth Factor, alpha (TGFA))
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抗原表位
- C-Term
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适用
- 人, 大鼠, 小鼠, Cow
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This TGFA antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- A 17-amino acid synthetic peptide from the carboxyl-terminus of rat TGF alpha was used as the immunogen for this antibody.
- 克隆位点
- MF9
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product TGFA Primary Antibody
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- 应用备注
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TGF alpha antibody to be titered up or down for optimal performance.
1. Staining of FFPE tissues requires boiling sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.\. Western blot: 1-2 μg/mL, FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (FFPE): 1-2 μg/mL for 30 min at RT (1)
- 限制
- 仅限研究用
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- 浓度
- 0.2 mg/mL
- 缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store the TGF alpha antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- 抗原
- TGFA (Transforming Growth Factor, alpha (TGFA))
- 别名
- TGF alpha (TGFA 产品)
- 别名
- TFGA antibody, LOC100009482 antibody, TGF alpha antibody, TGFA antibody, wa-1 antibody, wa1 antibody, RATTGFAA antibody, TGFAA antibody, NV15606 antibody, si:ch211-10e8.7 antibody, transforming growth factor alpha antibody, transforming growth factor alpha L homeolog antibody, transforming growth factor-alpha antibody, transforming growth factor, alpha antibody, TGFA antibody, TGF-A antibody, Tgfa antibody, tgfa.L antibody, LOC100121300 antibody, LOC100009150 antibody, tgfa antibody
- 背景
- TGF alpha is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. Staining with the antibody is completely blocked by the peptide used for raising this antibody.
- 基因ID
- 7039
- 途径
- NF-kappaB Signaling, RTK signaling, EGFR Signaling Pathway
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