p53 抗体 (N-Term)
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Quick Overview for p53 抗体 (N-Term) (ABIN3025364)
抗原
See all p53 (TP53) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
克隆位点
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抗原表位
- AA 20-25, N-Term
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原理
- Anti-p53 Antibody (azide and preservative free)
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无交叉反应
- 小鼠, 大鼠
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纯化方法
- Protein G affinity chromatography
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免疫原
- Recombinant human wild type p53 protein was used as the immunogen for this anti-p53 antibody. Its epitope maps within the N-terminus (approx. amino acids 20-25).
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亚型
- IgG2a, kappa
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应用备注
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The optimal dilution of the anti-p53 antibody for each application should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the anti-p53 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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- p53 (TP53) (Tumor Protein P53 (TP53))
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别名
- p53
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背景
- P53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis.
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UniProt
- P04637
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途径
- p53 Pathway, MAPK Pathway, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect
抗原
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