Actin 抗体
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- 抗原 See all Actin (ACTA1) 抗体
- Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))
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适用
- 人, 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Actin antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- An amino acid sequence common among human muscle actins was used as the immunogen for the Muscle Actin antibody.
- 克隆位点
- PMAC1-1
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product ACTA1 Primary Antibody
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anti-Actin, alpha 1, Skeletal Muscle (ACTA1) (AA 277-308), (C-Term) antibody
ACTA1 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Actin, alpha 1, Skeletal Muscle (ACTA1) antibodyACTA1 适用: 人, 大鼠, 兔, 斑马鱼, Pig, 小鸡, 猴 WB, ELISA, IHC, IHC (p), IHC (fro), IEM 宿主: 小鼠 Monoclonal HHF35 unconjugated
anti-Actin, alpha 1, Skeletal Muscle (ACTA1) (AA 346-375) antibodyACTA1 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Actin, alpha 1, Skeletal Muscle (ACTA1) (AA 3-377) antibodyACTA1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Actin, alpha 1, Skeletal Muscle (ACTA1) antibodyACTA1 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 兔 Monoclonal 25E3 unconjugated Recombinant Antibody
anti-Actin, alpha 1, Skeletal Muscle (ACTA1) (AA 1-129) antibodyACTA1 适用: 人, 斑马鱼, 鱼 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Actin, alpha 1, Skeletal Muscle (ACTA1) (AA 346-375), (C-Term) antibodyACTA1 适用: 人 WB, IHC (p), IF, FACS 宿主: 兔 Polyclonal RB35391 unconjugated
anti-Actin, alpha 1, Skeletal Muscle (ACTA1) antibodyACTA1 适用: 人 WB, IHC (p), FACS 宿主: 小鼠 Monoclonal 7C1 unconjugated
anti-Actin, alpha 1, Skeletal Muscle (ACTA1) (AA 7-377) antibodyACTA1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Actin, alpha 1, Skeletal Muscle (ACTA1) antibodyACTA1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Titering of the Muscle Actin antibody may be required for optimal performance.
1. FFPE testing requires sections to be boiled in pH 6 10 mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 0.5-1 μg/mL,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2) - 限制
- 仅限研究用
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- 浓度
- 0.2 mg/mL
- 缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store the Muscle Actin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- 抗原
- Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))
- 别名
- Muscle Actin (ACTA1 产品)
- 别名
- ACTA antibody, ASMA antibody, CFTD antibody, CFTD1 antibody, CFTDM antibody, MPFD antibody, NEM1 antibody, NEM2 antibody, NEM3 antibody, acta antibody, asma antibody, cftd antibody, cftd1 antibody, cftdm antibody, mpfd antibody, nem1 antibody, AA959943 antibody, Acta-2 antibody, Acts antibody, Actsk-1 antibody, actc antibody, actc1 antibody, cfk antibody, hm:zeh0631 antibody, zeh0631 antibody, acta1 antibody, hm:zeh0293 antibody, zgc:112098 antibody, actin, alpha 1, skeletal muscle antibody, actin, alpha 1, skeletal muscle L homeolog antibody, actin, alpha 1b, skeletal muscle antibody, actin, muscle antibody, actin, alpha skeletal muscle antibody, actin, alpha 1a, skeletal muscle antibody, ACTA1 antibody, acta1.L antibody, acta1 antibody, Acta1 antibody, acta1b antibody, LOC100533357 antibody, LOC109479779 antibody, LOC109108280 antibody, acta1a antibody
- 背景
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Actin participates in many important cellular processes, including muscle contraction, cell motility, cell division and cytokinesis, vesicle and organelle movement, cell signaling, and the establishment and maintenance of cell junctions and cell shape. An actin protein's mass is roughly 42- kDa and it is the monomeric subunit of two types of filaments in cells: microfilaments, one of the three major components of the cytoskeleton, and thin filaments, part of the contractile apparatus in muscle cells. It can be present as either a free monomer called G-actin (globular) or as part of a linear polymer microfilament called F-actin (filamentous), both of which are essential for such important cellular functions as the mobility and contraction of cells during cell division. [Wiki]
This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells.
- 途径
- Caspase Cascade in Apoptosis, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development
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