-
- 抗原 See all HLAG 抗体
- HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))
-
抗原表位
- C-Term
-
适用
- 人
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This HLAG antibody is conjugated to FITC
-
应用范围
- Flow Cytometry (FACS)
- 特异性
- The antibody 2A12 was generated to a peptide corresponding to C-intron 4-encoded sequence. This antibody does not crossreact with the full-length HLA-G1 isoform and thus allows to distinguish between secreted HLA-G5 and HLA-G6 isoforms from shedded HLA-G1.
- 交叉反应 (详细)
- Human
- 纯化方法
- Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
- 免疫原
- C-terminal amino acid sequence (22-mer) of soluble HLA-G5 and HLA-G6 proteins coupled to ovalbumin.
- 克隆位点
- 2A12
- 亚型
- IgG1
- Top Product
- Discover our top product HLAG Primary Antibody
-
-
- 应用备注
- Flow cytometry: Excellent for intracellular staining, recommended dilution: 1-5 μg/mL.
- 说明
-
The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.
- 限制
- 仅限研究用
-
- 浓度
- 1 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
-
Do not freeze.
Avoid prolonged exposure to light. - 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
-
-
HLA-G5 in the blood of leukemia patients and healthy individuals." in: Leukemia research, Vol. 37, Issue 2, pp. 139-45, (2013) (PubMed).
: "Human leukocyte antigen-G in the male reproductive system and in seminal plasma." in: Molecular human reproduction, Vol. 17, Issue 12, pp. 727-38, (2011) (PubMed).
: "Levels of soluble human leukocyte antigen-G are increased in asthmatic airways." in: The European respiratory journal, Vol. 35, Issue 4, pp. 925-7, (2010) (PubMed).
: "Biology and functions of human leukocyte antigen-G in health and sickness." in: Tissue antigens, Vol. 62, Issue 4, pp. 273-84, (2003) (PubMed).
: "
-
HLA-G5 in the blood of leukemia patients and healthy individuals." in: Leukemia research, Vol. 37, Issue 2, pp. 139-45, (2013) (PubMed).
-
- 抗原
- HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))
- 别名
- HLA-G (HLAG 产品)
- 别名
- MHC-G antibody, B-F antibody, B-F-S04 antibody, B-F-S05 antibody, B-F-S06 antibody, B-F-S07 antibody, B-FI antibody, B-FIV antibody, BF2 antibody, BFa2 antibody, BFw-03 antibody, BFw-05 antibody, BFz-01 antibody, major histocompatibility complex, class I, G antibody, MHC BF1 class I antibody, HLA-G antibody, BF1 antibody
- 背景
- Major histocompatibility complex, class I, G,Human leukocyte antigen G (HLA-G), belonging to MHC class I glycoproteins, plays important roles in both physiological and pathological immunotolerance. It gives an inhibitory signal to cytotoxic T cells, NK cells, monocytes, and some other immune cells. It also induces regulatory T cells and anti-inflammatory macrophages. HLA-G is important e.g. for maternal tolerance to the fetus, and for immunomodulation in particular adult tissues, such as in cornea, pancreatic islets, thymus and other. On the other hand, it is expressed in many solid and hematologic malignancies, where it contributes to evasion of the immune surveillance. HLA-G expression pattern in cancer is an important prognostic factor regarding a poor clinical outcome. Unlike most other MHC glycoproteins, HLA-G acts as an immune checkpoint molecule rather than as an antigen presenting molecule. It concerns both transmembrane and soluble HLA-G isoforms. Among other, HLA-G can promote Th2 immunological response and downregulate Th1 immunological response. For its benefits regarding allograft tolerance, including embryo implantation, soluble HLA-G (sHLA-G) can be used as a marker of developmental potential of embryos during the process of in vitro fertilization. Similarly, sHLA-G concentrations in maternal serum are decreased in preeclampsia. Transplanted patients with increased sHLA-G serum levels have improved allograft acceptance. On the other hand, increased sHLA-G can also indicate presence of malignant (sometimes also of benign) tumor cells. Another important topic is induction of HLA-G expression (sometimes associated with shedding of HLA-G from the cell surface) by some anti-cancer or anti-viral therapies, which can weaken the therapy effect. Monitoring of HLA-G in patients thus has a wide usage.
- 基因ID
- 3135
- UniProt
- P17693
- 途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints
-