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RBPJ 抗体 (AA 208-500)

RBPJ 适用: 人 WB, ELISA, ICC 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN295799
发货至: 中国
  • 抗原 See all RBPJ 抗体
    RBPJ (Recombination Signal Binding Protein For Immunoglobulin kappa J Region (RBPJ))
    抗原表位
    • 15
    • 8
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    • 6
    • 6
    • 4
    • 2
    • 2
    • 1
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    • 1
    • 1
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    AA 208-500
    适用
    • 74
    • 31
    • 15
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
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    宿主
    • 69
    • 3
    • 2
    • 1
    山羊
    克隆类型
    • 62
    • 13
    多克隆
    标记
    • 33
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
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    This RBPJ antibody is un-conjugated
    应用范围
    • 55
    • 28
    • 23
    • 13
    • 13
    • 9
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
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    Western Blotting (WB), ELISA, Immunocytochemistry (ICC)
    特异性
    Selected for its ability to recognize human CSL in the applications listed.
    纯化方法
    Affinity purified
    免疫原
    E. coli-derived rhCSL (aa208-500).
    亚型
    IgG
    Top Product
    Discover our top product RBPJ Primary Antibody
  • 应用备注
    Approved: ELISA, ICC, WB

    Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human CSL. The detection limit for rhCSL is approximately 10 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: This antibody can be used at a concentration of 10 μg/mL to detect human CSL on HASMC (human aortic smooth muscle) cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by NorthernLights 557-coupled donkey anti-goat IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human CSL. The detection limit for rhCSL is approximately 5 ng/well.
    说明

    Target Species of Antibody: Human

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    deionized water
    浓度
    Lot specific
    缓冲液
    Lyophilized.
  • 抗原
    RBPJ (Recombination Signal Binding Protein For Immunoglobulin kappa J Region (RBPJ))
    别名
    RBPJ (RBPJ 产品)
    别名
    AOS3 antibody, CBF1 antibody, IGKJRB antibody, IGKJRB1 antibody, KBF2 antibody, RBP-J antibody, RBPJK antibody, RBPSUH antibody, SUH antibody, csl antibody, AI843960 antibody, Igkjrb antibody, Igkrsbp antibody, RBP-J kappa antibody, RBPjk antibody, Rbpsuh antibody, Su(H) antibody, XSu(H) antibody, xsu(h)1 antibody, Su(H)1 antibody, Su(h)A antibody, ik:tdsubc_1e8 antibody, rbpj antibody, rbpsuh antibody, xx:tdsubc_1e8 antibody, Su(H)2 antibody, su(h)B antibody, recombination signal binding protein for immunoglobulin kappa J region antibody, recombination signal binding protein for immunoglobulin kappa J region S homeolog antibody, recombination signal binding protein for immunoglobulin kappa J region a antibody, recombination signal binding protein for immunoglobulin kappa J region b antibody, RBPJ antibody, Rbpj antibody, rbpj.S antibody, rbpja antibody, rbpjb antibody
    背景
    Name/Gene ID: RBPJ

    Synonyms: RBPJ, AOS3, CBF1, Csl, KBF2, H-2K binding factor-2, RBP-JK, SUH, Suppressor of hairless homolog, RBP-J, RBP-J kappa, RBPJK, RBPSUH, CBF-1, IGKJRB, IGKJRB1
    基因ID
    3516
    UniProt
    Q06330
    途径
    Notch Signaling, Stem Cell Maintenance, Smooth Muscle Cell Migration
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