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CHEK1 抗体 (pSer345)

Name: CHEK1 抗体 (pSer345)
Brand: antibodies-online
SKU: ABIN2857048
Availability: OutOfStock

This anti-CHEK1 antibody is a 兔多克隆 antibody detecting CHEK1 in WB, IF, IHC (p) 和 ICC. Suitable for 人. This Primary Antibody has been cited in 1 publication.

WB Image Sample: 20 ug of HCT116 P53 whole cell lysate 10% SDS PAGE Chk1-phopho-S345 antibody antibody diluted at 1:1000 (CHEK1 抗体 (pSer345))

IHC-P Image Chk1 (phospho Ser345) antibody [C1C2], Internal detects Chk1 (phospho Ser345) protein at nucleus on mouse colon by immunohistochemical analysis. (CHEK1 抗体 (pSer345))

IHC

ICC/IF Image Chk1 (phospho Ser345) antibody [C1C2], Internal detects Chk1 (phospho Ser345) protein at nucleus by immunofluorescent analysis. (CHEK1 抗体 (pSer345))

IHC-P Image Immunohistochemical analysis of paraffin-embedded HeLa xenograft, using Chk1 (phospho Ser345) , antibody at 1:500 dilution. (CHEK1 抗体 (pSer345))

IHC

4 images

(1 reference)

产品编号 ABIN2857048

发货至: 中国

Quick Overview for CHEK1 抗体 (pSer345) (ABIN2857048)

抗原

See all CHEK1 抗体

CHEK1 (Checkpoint Kinase 1 (CHEK1))

适用

人

宿主

兔

克隆类型

多克隆

标记

This CHEK1 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Discover our top product CHEK1 Primary Antibody

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pSer345

交叉反应

人, 小鼠

产品特性

Rabbit Polyclonal antibody to Chk1 (phospho Ser345) (CHK1 checkpoint homolog (S. pombe))
Chk1 (phospho Ser345) antibody [C1C2], Internal

纯化方法

Purified by antigen-affinity chromatography.

免疫原

Carrier-protein conjugated synthetic peptide surrounding phospho Ser345 of human Chk1. The exact sequence is proprietary.

亚型

IgG
anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibody
CHEK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Checkpoint Kinase 1 (CHEK1) (pSer301) antibody
CHEK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Checkpoint Kinase 1 (CHEK1) (pSer296) antibody
CHEK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Checkpoint Kinase 1 (CHEK1) (pSer286) antibody
CHEK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Checkpoint Kinase 1 (CHEK1) (Middle Region) antibody
CHEK1 适用: 人, 小鼠, 大鼠, 犬, 兔, Cow, 马, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibody
CHEK1 适用: 小鼠, 小鸡 ELISA, IHC (p), ICC, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Checkpoint Kinase 1 (CHEK1) antibody
CHEK1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Monoclonal 2F2 unconjugated Recombinant Antibody

anti-Checkpoint Kinase 1 (CHEK1) antibody
CHEK1 适用: 人, 小鼠 WB, ELISA 宿主: 小鼠 Monoclonal 2G1D5 unconjugated

anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibody
CHEK1 适用: 人 WB, IHC (p), DB 宿主: 兔 Polyclonal RB7845-RB18425 unconjugated

anti-Checkpoint Kinase 1 (CHEK1) (pSer296) antibody
CHEK1 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

应用备注

WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

说明

Positive Control: HCT116 cells with 30uM cisplatin treatment for 24hr , HCT116 P53 cell
Validation: Orthogonal

限制

仅限研究用
状态

Liquid

浓度

1.66 mg/mL

缓冲液

1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Xu, Zhou, Xiong, Zou, Deng, Ganaie, Kleiboeker, Peng, Liu, Wang, Ye, Qiu: "Parvovirus B19 NS1 protein induces cell cycle arrest at G2-phase by activating the ATR-CDC25C-CDK1 pathway." in: PLoS pathogens, Vol. 13, Issue 3, pp. e1006266, (2017) (PubMed).
抗原

CHEK1 (Checkpoint Kinase 1 (CHEK1))

别名

checkpoint kinase 1

背景

Required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Inhibition of CDC25 activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Binds to and phosphorylates RAD51 at 'Thr-309', which may enhance the association of RAD51 with chromatin and promote DNA repair by homologous recombination. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may affect chromatin assembly during S phase or DNA repair. May also phosphorylate multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and enhances suppression of cellular proliferation.

Cellular Localization: Nucleus , Cytoplasm

分子量

54 kDa

基因ID

1111

UniProt

O14757

途径

p53 Pathway, Apoptosis, Cell Division Cycle, DNA Damage Repair