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HRAS 抗体 (C-Term)

Name: HRAS 抗体 (C-Term)
Brand: antibodies-online
SKU: ABIN2857002
Availability: OutOfStock

Cited in 4+ publications. This 兔多克隆 anti-HRAS antibody specifically detects HRAS in WB, IF, ICC 和 IHC (p). The antibody is reactive with 人, 小鼠和大鼠 samples.

WB Image Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with H-Ras antibody , diluted at 1:5000. (HRAS 抗体 (C-Term))

IHC-P Image H-Ras antibody detects H-Ras protein at membrane on mouse intestine by immunohistochemical analysis. (HRAS 抗体 (C-Term))

IHC

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using H-Ras , antibody at 1:200 dilution. (HRAS 抗体 (C-Term))

WB Image Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 , C: Molt-4 , 12% SDS PAGE antibody diluted at 1:1000 (HRAS 抗体 (C-Term))

IHC-P Image H-Ras antibody detects H-Ras protein at membrane on mouse prostate by immunohistochemical analysis. (HRAS 抗体 (C-Term))

IHC

WB Image H-Ras antibody detects HRAS protein by Western blot analysis. (HRAS 抗体 (C-Term))

mRNA expression (all n = 6) of HRas, MAPK14 (p38), CCL2, DOK1 and PTK2B related to GAPDH mRNA expression, protein expression of HRas (n = 6), p38 (n = 6), CCL2 (n = 4), DOK1 (n = 7-8) and PTK2B (n = 3) related to GAPDH expression in A549 () and A549rCDDP2000 () before (ctrl) and after treatment with 11 μM cisplatin (11) or 34 μM cisplatin (34) presented as mean ± SEM, as well as representative Western blots. (HRAS 抗体 (C-Term))

Overexpression of Nogo-B receptor (NgBR) increases the resistance of MCF-7 cells to tamoxifen. (HRAS 抗体 (C-Term))

8 images

(4 references)

产品编号 ABIN2857002

发货至: 中国

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Quick Overview for HRAS 抗体 (C-Term) (ABIN2857002)

抗原

See all HRAS 抗体

HRAS (HRas proto-oncogene, GTPase (HRAS))

适用

人, 小鼠, 大鼠

宿主

兔

克隆类型

多克隆

标记

This HRAS antibody is un-conjugated

应用范围

Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

抗原表位
15

15

8

8

6

5

4

3

3

2

2

2

2

2

1

1

1

1

1
C-Term

产品特性

Rabbit Polyclonal antibody to H-Ras (v-Ha-ras Harvey rat sarcoma viral oncogene homolog)
H-Ras antibody

纯化方法

Purified by antigen-affinity chromatography.

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human H-Ras.
anti-HRas proto-oncogene, GTPase (HRAS) antibody
HRAS 适用: 人, 小鼠, 大鼠, 犬, 猴 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 1C2 unconjugated

anti-HRas proto-oncogene, GTPase (HRAS) (AA 104-128) antibody
HRAS 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-HRas proto-oncogene, GTPase (HRAS) antibody
HRAS 适用: 人, 小鼠, 大鼠, 犬, 猴 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 1A1 unconjugated

anti-HRas proto-oncogene, GTPase (HRAS) (AA 104-128) antibody
HRAS 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB15622 unconjugated

anti-HRas proto-oncogene, GTPase (HRAS) (AA 146-176) antibody
HRAS 适用: 人, 小鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-HRas proto-oncogene, GTPase (HRAS) antibody
KD Validated HRAS 适用: 人 WB, ICC, FACS 宿主: 兔 Monoclonal 23GB6035 unconjugated Recombinant Antibody

anti-HRas proto-oncogene, GTPase (HRAS) antibody
KD Validated HRAS 适用: 人 WB 宿主: 小鼠 Monoclonal 24GB5695 unconjugated

anti-HRas proto-oncogene, GTPase (HRAS) (AA 1-189) antibody
HRAS 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-HRas proto-oncogene, GTPase (HRAS) (AA 12-181) antibody
HRAS 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-HRas proto-oncogene, GTPase (HRAS) (AA 2-186) antibody
HRAS 适用: 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

应用备注

WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000.
Not yet tested in other applications.
Optimal working dilutions should be determined experimentally by the end user.

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

0.1M Tris, 0.1M Glycine, 20 % Glycerol ( pH 7).
0.01 % Thimerosal was added as a preservative.

储存液

Thimerosal (Merthiolate)

注意事项

This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Keep as concentrated solution. Aliquot and store at -20°C or below.
Avoid multiple freeze-thaw cycles.
Basu, Lee, Salotti, Basu, Sakchaisri, Xiao, Walia, Westlake, Morrison, Johnson: "Oncogenic RAS-Induced Perinuclear Signaling Complexes Requiring KSR1 Regulate Signal Transmission to Downstream Targets." in: Cancer research, Vol. 78, Issue 4, pp. 891-908, (2019) (PubMed).

Brandt, Albers, Hejhal, Pfundstein, Gonçalves, Catalano, Wild, Frew: "Mouse genetic background influences whether HrasG12V expression plus Cdkn2a knockdown causes angiosarcoma or undifferentiated pleomorphic sarcoma." in: Oncotarget, Vol. 9, Issue 28, pp. 19753-19766, (2018) (PubMed).

Gao, Wang, Jin, Hu, Duan, Shi, Du, Song, Yang, Li, Han, Zhao, Zhang, Fan, Miao: "Nogo-B receptor increases the resistance to tamoxifen in estrogen receptor-positive breast cancer cells." in: Breast cancer research : BCR, Vol. 20, Issue 1, pp. 112, (2018) (PubMed).

Brandt, Albers, Hejhal, Catalano, Wild, Frew: "Oncogenic HrasG12V expression plus knockdown of Cdkn2a using ecotropic lentiviral vectors induces high-grade endometrial stromal sarcoma." in: PLoS ONE, Vol. 12, Issue 10, pp. e0186102, (2017) (PubMed).
抗原

HRAS (HRas proto-oncogene, GTPase (HRAS))

别名

H-Ras

基因ID

3265

途径

p53 Pathway, MAPK Pathway, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Autophagy, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling, BCR Signaling