CD45 抗体
(17 references)-
- 抗原 See all CD45 (PTPRC) 抗体
- CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
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适用
- 人, 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This CD45 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))
- 品牌
- BD Pharmingen™
- 产品特性
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The rat anti-mouse CD45R antibody (clone RA3-6B2) has been reported to react with an epitope on the extracellular domain of the CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. It is expressed on B lymphocytes at all stages from pro-B through mature and activated B cell, but it is decreased on plasma cells and a subset of memory B cells. Levels of expression of CD45R on the B-cell lineage appear to be developmentally regulated. It is also reportedly found on the abnormal T cells involved in the lymphadenopathy of lpr/lpr and gld/gld mutant mice, on lytically active subsets of lymphokine-activated killer cells (NK cells and non-MHC-restricted CTL), on apoptotic T lymphocytes of mice injected with bacterial superantigen, on a population of NK-cell precursors in the bone marrow, and on B-lymphocyte, T-lymphocyte, and macrophage progenitors in fetal liver. The CD45R antigen has been reported not to be on hematopoietic stem cells, naive T lymphocytes, or MHC-restricted CTL. CD45 is a member of the Protein Tyrosine Phosphatase (PTP) family: Its intracellular (COOH-terminal) region contains two PTP catalytic domains, and the extracellular region is highly variable due to alternative splicing of exons 4, 5, and 6 (designated A, B, and C, respectively), plus differing levels of glycosylation. The CD45 isoforms detected in the mouse are cell type-, maturation, and activation state-specific. The CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction. CD45R is commonly used as a pan B-cell marker, however, CD19 expression, detectable by the rat anti-mouse CD19 antibody (clone 1D3), is reported to be more restricted to the B-cell lineage. The rat anti-mouse CD45R antibody (clone RA3-6B2) has been reported to enhance isotype switching during in vitro B-cell responses and to inhibit in vivo B-cell responses. Cross-reaction of the RA3-6B2 clone with activated human T lymphocytes has also been reportedly observed. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.
BD Pharmingen™ Purified Rat Anti-Mouse CD45R - Purified - Clone RA3-6B2 - Isotype Rat IgG2a, κ - Reactivity Ms, Hu - 0.1 mg - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Abelson murine leukemia virus (MuLV) induced pre-B tumor Cells
- 克隆位点
- RA3
- 亚型
- IgG2a kappa
- Top Product
- Discover our top product PTPRC Primary Antibody
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anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) (AA 1210-1304) antibody
PTPRC 适用: 人, 小鼠, 大鼠, 兔 FACS, WB, IHC (p), ELISA, IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) antibodyPTPRC 适用: 人, 犬 FACS, IHC, WB, StM 宿主: 小鼠 Monoclonal 2B11-PD7-26 unconjugated
anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) (AA 193-575) antibodyPTPRC 适用: 人 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) (AA 371-556) antibodyPTPRC 适用: 小鼠 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) (AA 24-575) antibodyPTPRC 适用: 人 FACS, IHC, WB, IF, ELISA 宿主: 小鼠 Monoclonal 17H9D4 unconjugated
anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) (C-Term) antibodyPTPRC 适用: 人, 小鼠, 大鼠, 犬, 小鸡, Cow, Cat, 兔, 绵羊, Pig, 豚鼠, 山羊, 马, 猴, Avian, 驴, Hamster WB 宿主: 山羊 Polyclonal unconjugated
anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) antibodyPTPRC 适用: 人 FACS, IHC, WB, IF, StM 宿主: 兔 Monoclonal PTPRC-2877R unconjugated Recombinant Antibody
anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) antibodyPTPRC 适用: 人 FACS, WB, IP, IHC (p), ICC 宿主: 小鼠 Monoclonal MEM-28 unconjugated
anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) (AA 378-577) antibodyPTPRC 适用: 人, 小鼠, 大鼠 FACS, IHC, WB, ELISA, ICC 宿主: 小鼠 Monoclonal 2H3E2 unconjugated
anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) (AA 431-577) antibodyPTPRC 适用: 人 FACS, IHC, WB, ELISA, ICC, Neut 宿主: 小鼠 Monoclonal 7C9F12 unconjugated
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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: "Human T cell activation induces the expression of a novel CD45 isoform that is analogous to murine B220 and is associated with altered O-glycan synthesis and onset of apoptosis." in: Cellular immunology, Vol. 213, Issue 1, pp. 72-81, (2001) (PubMed).
: "Development and maintenance of a B220- memory B cell compartment." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 3, pp. 1393-405, (2001) (PubMed).
: "Apoptosis with FasL+ cell infiltration in the periphery and thymus of corrected autoimmune mice." in: Immunology, Vol. 92, Issue 2, pp. 206-13, (1998) (PubMed).
: "B220 expression by T lymphoid progenitor cells in mouse fetal liver." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 2, pp. 666-76, (1997) (PubMed).
: "Self-renewal of B-1 lymphocytes is dependent on CD19." in: European journal of immunology, Vol. 26, Issue 1, pp. 238-42, (1996) (PubMed).
: "IL-15 can substitute for the marrow microenvironment in the differentiation of natural killer cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 10, pp. 4282-5, (1996) (PubMed).
: "Peripheral T cells undergoing superantigen-induced apoptosis in vivo express B220 and upregulate Fas and Fas ligand." in: The Journal of experimental medicine, Vol. 183, Issue 2, pp. 431-7, (1996) (PubMed).
: "A subpopulation of B220+ cells in murine bone marrow does not express CD19 and contains natural killer cell progenitors." in: The Journal of experimental medicine, Vol. 183, Issue 1, pp. 187-94, (1996) (PubMed).
: "Administration of anti-CD45 mAb specific for a B cell-restricted epitope abrogates the B cell response to a T-dependent antigen in vivo." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 11, pp. 5936-47, (1994) (PubMed).
: "Ligation of CD45 on B cells can facilitate production of secondary Ig isotypes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 3, pp. 1014-21, (1994) (PubMed).
: "In vivo CD4+ lymph node T cells from lpr mice generate CD4-CD8-B220+TCR-beta low cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 9, pp. 3948-55, (1994) (PubMed).
: "Lymphokine-activated killer cells. VII. IL-4 induces an NK1.1+CD8 alpha+beta- TCR-alpha beta B220+ lymphokine-activated killer subset." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 1, pp. 17-30, (1993) (PubMed).
: "Peripheral B cell maturation. I. Immature peripheral B cells in adults are heat-stable antigenhi and exhibit unique signaling characteristics." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 8, pp. 2533-40, (1992) (PubMed).
: "CD45 expression by B cells. Expression of different CD45 isoforms by subpopulations of activated B cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 7, pp. 2286-94, (1992) (PubMed).
: "Resolution and characterization of pro-B and pre-pro-B cell stages in normal mouse bone marrow." in: The Journal of experimental medicine, Vol. 173, Issue 5, pp. 1213-25, (1991) (PubMed).
: "Treatment of autoimmune MRL/lpr mice with anti-B220 monoclonal antibody reduces the level of anti-DNA antibodies and lymphadenopathies." in: Immunology, Vol. 68, Issue 2, pp. 204-8, (1989) (PubMed).
: "Surface antigen expression and immunoglobulin gene rearrangement during mouse pre-B cell development." in: Immunological reviews, Vol. 69, pp. 5-23, (1983) (PubMed).
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: "Human T cell activation induces the expression of a novel CD45 isoform that is analogous to murine B220 and is associated with altered O-glycan synthesis and onset of apoptosis." in: Cellular immunology, Vol. 213, Issue 1, pp. 72-81, (2001) (PubMed).
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- 抗原
- CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
- 别名
- CD45R (PTPRC 产品)
- 别名
- PTPRC antibody, si:dkeyp-47c5.1 antibody, B220 antibody, CD45 antibody, CD45R antibody, GP180 antibody, L-CA antibody, LCA antibody, LY5 antibody, T200 antibody, Cd45 antibody, Ly-5 antibody, Lyt-4 antibody, loc antibody, Lca antibody, RT7 antibody, cd45 antibody, protein tyrosine phosphatase, receptor type C antibody, protein tyrosine phosphatase, receptor type, C antibody, receptor-type tyrosine-protein phosphatase C antibody, PTPRC antibody, ptprc antibody, LOC100074271 antibody, Ptprc antibody
- 背景
- Synonyms: B220
- 途径
- TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling
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