(8 references)-
- 抗原 See all TNFRSF1B 抗体
- TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))
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适用
- 人
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This TNFRSF1B antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS)
- 品牌
- BD Pharmingen™
- 产品特性
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The hTNFR-M1 antibody reacts with the extracellular domain of the 75 kDa transmembrane receptor for the human cytokines, tumor necrosis factor (TNF or TNF-α) and lymphotoxin-alpha (LT-α3, aka, lymphotoxin or TNF-β). This receptor is referred to as the p75 or Type II Tumor Necrosis Factor Receptor (TNFRII) [aka, CD120b]. Human TNFRII proteins are expressed by hematopoietic cells including macrophages, neutrophils, lymphocytes, thymocytes and mast cells. TNFRII is expressed by a variety of other cell types including endothelial cells, cardiac myocytes and prostate cells. Naive B cells express very low or undetectable levels of TNFRII whereas mature erythrocytes and platelets are uniformly negative for TNFRII expression. The immunogen used to generate the hTNFR-M1 hybridoma was COS- expressed recombinant human TNFRII. Expression of cell surface TNFRII by whole-lysed human blood. Whole human blood was lysed with PharmLyse™ (Cat. No. 555899) prior to staining with hTNFR-M1. Whole lysed human blood was subsequently blocked with normal polyclonal human IgG and stained with purified hTNFR-M1 (0.06 μg/10e6 cells, Cat No. 551311) followed by biotinylated goat anti-rat F(ab')2 IgG and streptavidin phycoerythrin (0.015 μg, Cat. No. 554061). Staining with the hTNFR-M1 antibody (filled histograms) is compared to staining obtained using the isotype control antibody (open histograms). The histograms in the figure were derived from gated events with the light scattering characteristics of viable lymphocytes (left panel), monocytes (center panel) and granulocytes (right panel). Note: Certain human cell lines or cell types (e.g., neutrophils, monocytes) can first be treated with reagents that block receptors for the Fc regions of immunoglobulin to avoid nonspecific immunofluorescent staining mediated by Fc receptors.
BD Pharmingen™ Purified Rat Anti-Human CD120b - Purified - Clone hTNFR-M1 - Isotype Rat IgG2b, κ - Reactivity Hu - 0.5 mg - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- COS-expressed recombinant human TNFRII
- 克隆位点
- HTNFR
- 亚型
- IgG2b kappa
- Top Product
- Discover our top product TNFRSF1B Primary Antibody
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anti-Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) (AA 23-257) antibody
TNFRSF1B 适用: 人, 小鼠 WB, ELISA, IHC, FACS, ICC, Neut 宿主: 小鼠 Monoclonal 2H11C2 unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) (AA 23-257) antibodyTNFRSF1B 适用: 人 WB, ELISA, IHC, FACS, ICC, Neut 宿主: 小鼠 Monoclonal 7G8B6 unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) antibodyTNFRSF1B 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) (C-Term) antibodyTNFRSF1B 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) (N-Term) antibodyTNFRSF1B 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) (AA 30-250) antibodyTNFRSF1B 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) (C-Term) antibodyTNFRSF1B 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) (AA 1-461) antibodyTNFRSF1B 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) antibodyTNFRSF1B 适用: 人 WB, ELISA, IHC, IHC (p), Neut 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) antibodyTNFRSF1B 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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: "Increased TNF-alpha-induced apoptosis in lymphocytes from aged humans: changes in TNF-alpha receptor expression and activation of caspases." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 4, pp. 2154-61, (1999) (PubMed).
: "Characterization of surface lymphotoxin forms. Use of specific monoclonal antibodies and soluble receptors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 1, pp. 33-46, (1995) (PubMed).
: "Both tumor necrosis factor receptor types mediate proliferative signals in human mononuclear cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 3, pp. 911-7, (1992) (PubMed).
: "Soluble and cell surface receptors for tumor necrosis factor." in: Agents and actions. Supplements, Vol. 35, pp. 51-7, (1992) (PubMed).
: "Two types of TNF receptors are expressed on human normal and malignant B lymphocytes." in: Clinical immunology and immunopathology, Vol. 61, Issue 2 Pt 1, pp. 260-7, (1991) (PubMed).
: "Identification of two types of tumor necrosis factor receptors on human cell lines by monoclonal antibodies." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 8, pp. 3127-31, (1990) (PubMed).
: "Two different cell types have different major receptors for human tumor necrosis factor (TNF alpha)." in: The Journal of biological chemistry, Vol. 264, Issue 25, pp. 14927-34, (1989) (PubMed).
: "Tumor necrosis factor: receptors on hematopoietic cells." in: Blood, Vol. 70, Issue 6, pp. 1730-4, (1988) (PubMed).
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: "Increased TNF-alpha-induced apoptosis in lymphocytes from aged humans: changes in TNF-alpha receptor expression and activation of caspases." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 4, pp. 2154-61, (1999) (PubMed).
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- 抗原
- TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))
- 别名
- CD120b (TNFRSF1B 产品)
- 别名
- CD120b antibody, TBPII antibody, TNF-R-II antibody, TNF-R75 antibody, TNFBR antibody, TNFR1B antibody, TNFR2 antibody, TNFR80 antibody, p75 antibody, p75TNFR antibody, TNF-RII/TNFRSF1B antibody, TNFR-II antibody, TNFRSF1B antibody, TNF-R2 antibody, TNF-alphaR2 antibody, TNFRII antibody, TNFalpha-R2 antibody, Tnfr-1 antibody, Tnfr2 antibody, rank antibody, zgc:163064 antibody, TNF receptor superfamily member 1B antibody, tumor necrosis factor receptor superfamily, member 1b antibody, tumor necrosis factor receptor superfamily, member 1B antibody, TNFRSF1B antibody, Tnfrsf1b antibody, tnfrsf1b antibody
- 背景
- Synonyms: TNF Receptor Type II
- 途径
- NF-kappaB Signaling, Apoptosis, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Ubiquitin Proteasome Pathway
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