Nestin antibody can be used in ELISA starting at 1:1000 - 1:6000, Western Blot, and immunohistochemistry starting at 5 μg/mL.
限制
仅限研究用
状态
Liquid
缓冲液
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
As with all antibodies avoid freeze/thaw cycles.
储存条件
4 °C/-20 °C
储存方法
Store Nestin antibody at 4 °C or -20 °C.
抗原
Nestin (NES)
别名
Nestin
背景
Nestin, is a large intermediate filament protein (class Type VI) that was first identified with a monoclonal antibody by Hockfield and McKay (1985). Nestin is expressed predominantly in stem cells of the central nervous system in the neural tube. Nestin is expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker, however, recent studies have found nestin expression in other cell types such as endothelial cells. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.