Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
特异性
This antibody is expected to recognize both reported isoforms according to NP_569079.1 and NP_064520.2.
纯化方法
WRNIP1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
WRNIP1 antibody was raised against a 14 amino acid synthetic peptide near the C-Terminus of WRNIP1.
ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 45 kDa band in human lung and brain lysates after 1 μg/mL antibody staining.2 and 69.5 kDa for isoform 2 according to NP_569079.1. The 45 kDa band was successfully blocked by incubation with the immunizing peptide. Approximately 90 kDa band observed in Jurkat lysate. an additional band of unknown identity was also consistently observed at 50 kDa . This band was successfully blocked by incubation with the immunizing peptide. IHC: In paraffin embedded Human Kidney shows nuclear staining, stronger in DCT compared to PCT. Recommended concentration, 4-6μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Minimize freezing and thawing.
储存条件
-20 °C
储存方法
Aliquot and store at -20 °C.
抗原
WRNIP1
(Werner Helicase Interacting Protein 1 (WRNIP1))