This antibody is expected to recognize both reported isoforms (NP_071320.1 and NP_071505.1).
纯化方法
TIA1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
TIA1 antibody was raised against an 11 amino acid synthetic peptide near the internal region of TIA1.
ELISA: Antibody detection limit dilution 1:128,000. Western Blot: Approximately 40 kDa band observed in Jurkat lysate (calculated MW of 41.8 kDa according to NP_071320.1). Recommended concentration: 0.1-0.3 μg/mL. an additional band at 80 kDa and a very strong 26 kDa is also observed. These bands were successfully blocked by incubation with the immunizing peptide.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Minimize freezing and thawing.
储存条件
-20 °C
储存方法
Aliquot and store at -20 °C.
抗原
TIA1
(TIA1 Cytotoxic Granule-Associated RNA Binding Protein (TIA1))