This antibody is expected to recognize both reported isoforms (NP_001018121.1 and NP_005388.2).
纯化方法
PODXL antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
PODXL antibody was raised against a 15 amino acid synthetic peptide near the C-Terminus of PODXL.
ELISA: Antibody detection limit dilution 1:64,000. Approximately 160 kDa band observed in human kidney and mouse kidney lysates (calculated MW of 58.6 kDa according to NP_001018121.1 and 55.4 kDa according to NP_005388.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Kershaw et al., J Biol Chem. 1997 Jun 20,272 (25) :15708-14., PMID: 9188463). Recommended concentration: 0.5-1.5 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.