PGRMC1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
PGRMC1 antibody was raised against an 11 amino acid synthetic peptide near the C-Terminus of PGRMC1.
ELISA: Antibody detection limit dilution 1:4000. Western Blot: Approximately 26 kDa band observed in human kidney, liver and testis lysates (calculated MW of 21.7 kDa according to NP_006658.1). In transfected HEK293 transiently expressing human PGRMC1 a band of approximately 26 kDa is observed. This band is not observed in the non-transfected HEK293. The endogenous 20 kDa protein is observed in both. Recommended concentration: 0.03-0.1 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.