This antibody is expected to recognize all three reported isoforms (NP_000823.4, NP_067544.1, NP_015566.1).
纯化方法
NMDA Receptor antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
NMDA Receptor antibody was raised against a 12 amino acid synthetic peptide near the internal region of NMDA Receptor.
ELISA: Antibody detection limit dilution 1:1,000. Western Blot: Approximately 110 kDa band observed in rat brain lysates (calculated MW of 105 kDa according to NP_015566.1). Recommended concentration: 2-4 μg/mL. A minor band of unknown identity was also consistently observed at 23-24 kDa . This band was strong in mouse brain lysates and faint in human brain lysates. The band was successfully blocked by incubation with the immunizing peptide.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.