Western Blotting (WB), Immunohistochemistry (IHC), ELISA
特异性
This antibody is expected to recognize an epitope corresponding to aa 1546-1560 of human LRP6 protein.
纯化方法
LRP6 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
LRP6 antibody was raised against a 15 amino acid synthetic peptide near the internal region of LRP6.
ELISA: Antibody detection limit dilution 1:64000. Western Blot: Approximately 50 kDa and 35 kDa in human adipose lysate after 0.1 μg/mL antibody staining.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). Immunohistochemistry: In paraffin embedded human kidney shows luminar membrane staining in DCT. Recommended concentration, 4-6 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Minimize freezing and thawing.
储存条件
-20 °C
储存方法
Aliquot and store at -20 °C.
抗原
LRP6
(Low Density Lipoprotein Receptor-Related Protein 6 (LRP6))