ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
纯化方法
FBXW2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
FBXW2 antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of FBXW2.
FBXW2
适用: 人
IF (cc), IF (p)
宿主: 兔
Polyclonal
Alexa Fluor 350
应用备注
ELISA: Antibody detection limit dilution 1:128000. Western Blot: Approximately 48 kDa band observed in human kidney lysates (calculated MW of 48.0 kDa according to NP_036296.1). Recommended concentration: 0.3-1.5 μg/mL. A stronger band of unknown identity was also consistently observed at appr. 30 kDa - both bands were successfully blocked by incubation with the immunizing peptide. Immunohistochemistry: In paraffin embedded human kidney, shows cytoplasmic staining of epithelial cells in some renal tubules. Recommended concentration, 2-3 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Minimize freezing and thawing.
储存条件
-20 °C
储存方法
Aliquot and store at -20 °C.
抗原
FBXW2
(F-Box and WD Repeat Domain Containing 2 (FBXW2))