Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
纯化方法
RAC2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
RAC2 antibody was raised against a 14 amino acid synthetic peptide near the C-Terminus of RAC2.
ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 20 kDa band observed in lysates of human tonsil lysate (calculated MW of 21.4 kDa according to NP_002863.1). Recommended concentration: 0.1-1 μg/mL. Immunohistochemistry: In paraffin embedded human tonsil, stains most cells in the mantle zone and interfollicular area. In the germinal centres the majority of cells are negative. Recommended concentration 0.3 μg/mL. IHC data were obtained with a previous batch.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Minimize freezing and thawing.
储存条件
-20 °C
储存方法
Aliquot and store at -20 °C.
抗原
RAC2
(Ras-Related C3 Botulinum Toxin Substrate 2 (Rho Family, Small GTP Binding Protein Rac2) (RAC2))